Plasma vitamin A analysis by liquid chromatography: Probucol interference

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dc.contributor.authorShephard G.S.
dc.contributor.authorHough B.J.
dc.contributor.authorVan Stuijvenberg M.E.
dc.contributor.authorLabadarios D.
dc.date.accessioned2011-05-15T16:16:04Z
dc.date.available2011-05-15T16:16:04Z
dc.date.issued1985
dc.description.abstractThe analysis of plasma retinol by high-performance liquid chromatography (HPLC) has recently become a widely accepted routine method. Many of the published reports involve reverse phase separations using methanol or methanol/water mixtures with ultraviolet (UV) detection. This experimental technique has the advantage of allowing simultaneous determinations of vitamin A (retinol) and vitamin E (α-tocopherol). The accuracy of all analytical chromatography methods is vitally dependent on the purity of the observed chromatographic peak. Constant awareness must be maintained for the presence of extraneous compounds which can coelute with the analyte of interest. This is of particular importance when dealing with pathological samples where the patients may be receiving drugs whose effect on the analysis is unknown. This note reports the interference of probucol (4,4'-(isopropylidenedithio)bis(2,6-di-t-butylphenol)) an anticholesterolaemic agent (Fig.1), on plasma vitamin A analysis.
dc.description.versionArticle
dc.identifier.citationClinica Chimica Acta
dc.identifier.citation153
dc.identifier.citation3
dc.identifier.issn00098981
dc.identifier.urihttp://hdl.handle.net/10019.1/13617
dc.subjectalpha tocopherol
dc.subjectprobucol
dc.subjectretinol
dc.subjectblood and hemopoietic system
dc.subjectdrug analysis
dc.subjectdrug blood level
dc.subjectdrug determination
dc.subjectdrug interaction
dc.subjecthuman
dc.subjecthuman cell
dc.subjectinterference
dc.subjectliquid chromatography
dc.subjectmethodology
dc.subjectplasma
dc.subjectpriority journal
dc.subjecttherapy
dc.subjectultraviolet spectrophotometry
dc.subjectChromatography, High Pressure Liquid
dc.subjectFalse Positive Reactions
dc.subjectHuman
dc.subjectPhenols
dc.subjectProbucol
dc.subjectVitamin A
dc.titlePlasma vitamin A analysis by liquid chromatography: Probucol interference
dc.typeArticle
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