Expression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood

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dc.contributor.authorJanse B.J.H.
dc.contributor.authorGaskell J.
dc.contributor.authorAkhtar M.
dc.contributor.authorCullen D.
dc.date.accessioned2011-05-15T15:56:34Z
dc.date.available2011-05-15T15:56:34Z
dc.date.issued1998
dc.description.abstractExpression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.The precise roles and interactions of these genes in lignin degradation and in commercial processes such as biomechanical pulping are poorly understood. Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation64
dc.identifier.citation9
dc.identifier.issn992240
dc.identifier.urihttp://hdl.handle.net/10019.1/9921
dc.subjectEnzymes
dc.subjectLignin
dc.subjectManganese compounds
dc.subjectRegression analysis
dc.subjectRNA
dc.subjectWood
dc.subjectBiomechanical pulping
dc.subjectGlyoxal oxidase
dc.subjectLignin peroxidases
dc.subjectMagnetic capture
dc.subjectManganese peroxidases
dc.subjectPhanerochaete chrysosporium
dc.subjectTranscript patterns
dc.subjectGenes
dc.subjectglyoxal oxidase
dc.subjectlignin peroxidase
dc.subjectmanganese peroxidase
dc.subjectmessenger RNA
dc.subjectpolyadenylated RNA
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectgene expression
dc.subjectmagnetic field
dc.subjectnonhuman
dc.subjectPhanerochaete
dc.subjectpolymerase chain reaction
dc.subjectquantitative diagnosis
dc.subjectsoil pollution
dc.subjectwood
dc.subjectActinobacteria (class)
dc.subjectChrysosporium
dc.subjectPhanerochaete
dc.subjectPhanerochaete chrysosporium
dc.subjectuncultured actinomycete
dc.titleExpression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood
dc.typeArticle
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