Phytase activity in Cryptococcus laurentii ABO 510

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dc.contributor.authorVan Staden J.
dc.contributor.authorDen Haan R.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorBotha A.
dc.contributor.authorViljoen-Bloom M.
dc.date.accessioned2011-05-15T16:03:26Z
dc.date.available2011-05-15T16:03:26Z
dc.date.issued2007
dc.description.abstractTen Cryptococcus strains were screened for phytase activity, of which the Cryptococcus laurentii ABO 510 strain showed the highest level of activity. The cell wall-associated enzyme displayed temperature and pH optima of 62°C and 5.0, respectively. The enzyme was thermostable at 70°C, with a loss of 40% of its original activity after 3 h. The enzyme was active on a broad range of substrates, including ATP, d-glucose 6-phosphate, d-fructose 1,6-diphosphate and p-nitrophenyl phosphate (p-NPP), but its preferred substrate was phytic acid (Km of 21 μM). The enzyme activity was completely inhibited by 0.5 mM inorganic phosphate or 5 mM phytic acid, and moderately inhibited in the presence of Hg2+, Zn2+, Cd2+ and Ca 2+. These characteristics suggest that the Cry. laurentii ABO 510 phytase may be considered for application as an animal feed additive to assist in the hydrolysis of phytate complexes to improve the bioavailability of phosphorus in plant feedstuff. © 2007 Federation of European Microbiological Societies.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation7
dc.identifier.citation3
dc.identifier.issn15671356
dc.identifier.other10.1111/j.1567-1364.2006.00196.x
dc.identifier.urihttp://hdl.handle.net/10019.1/12623
dc.subject4 nitrophenyl phosphate
dc.subjectadenosine triphosphate
dc.subjectcadmium
dc.subjectcalcium
dc.subjectfood additive
dc.subjectfructose
dc.subjectfungal enzyme
dc.subjectglucose 6 phosphate dehydrogenase
dc.subjectmercury
dc.subjectphosphate
dc.subjectphosphorus derivative
dc.subjectphytase
dc.subjectphytate
dc.subjectphytic acid
dc.subjectzinc ion
dc.subjectanimal food
dc.subjectarticle
dc.subjectbioavailability
dc.subjectcell wall
dc.subjectCryptococcus laurentii
dc.subjectenzyme activity
dc.subjectenzyme substrate
dc.subjectfungal strain
dc.subjecthydrolysis
dc.subjectnonhuman
dc.subjectpH
dc.subjectscreening
dc.subjecttemperature
dc.subjectthermostability
dc.subject6-Phytase
dc.subjectAnimal Feed
dc.subjectCell Wall
dc.subjectCryptococcus
dc.subjectEnzyme Stability
dc.subjectHydrogen-Ion Concentration
dc.subjectMetals, Heavy
dc.subjectPhosphates
dc.subjectPhytic Acid
dc.subjectSubstrate Specificity
dc.subjectAnimalia
dc.subjectCryptococcus laurentii
dc.titlePhytase activity in Cryptococcus laurentii ABO 510
dc.typeArticle
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