Seeing the forest through the (phylogenetic) trees : functional characterisation of grapevine terpene synthase (VviTPS) paralogues and orthologues

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dc.contributor.authorSmit, Samuel J.en_ZA
dc.contributor.authorVivier, Melane A.en_ZA
dc.contributor.authorYoung, Philip R.en_ZA
dc.contributor.editorNussbaumer, Thomasen_ZA
dc.contributor.editorSeetharam, Arumen_ZA
dc.date.accessioned2021-12-23T07:43:56Z
dc.date.available2021-12-23T07:43:56Z
dc.date.issued2021-07-26
dc.descriptionCITATION: Smit, S. J., Vivier, M. A. & Young, P. R. 2021. Seeing the forest through the (phylogenetic) trees : functional characterisation of grapevine terpene synthase (VviTPS) paralogues and orthologues. Plants, 10(8):1520, doi:10.3390/plants10081520.en_ZA
dc.descriptionThe original publication is available at https://www.mdpi.comen_ZA
dc.description.abstractGene families involved in specialised metabolism play a key role in a myriad of ecophysiological and biochemical functions. The Vitis vinifera sesquiterpene synthases represent the largest subfamily of grapevine terpene synthase (VviTPS) genes and are important volatile metabolites for wine flavour and aroma, as well as ecophysiological interactions. The functional characterisation of VviTPS genes is complicated by a reliance on a single reference genome that greatly underrepresents this large gene family, exacerbated by extensive duplications and paralogy. The recent release of multiple phased diploid grapevine genomes, as well as extensive whole-genome resequencing efforts, provide a wealth of new sequence information that can be utilised to overcome the limitations of the reference genome. A large cluster of sesquiterpene synthases, localised to chromosome 18, was explored by means of comparative sequence analyses using the publicly available grapevine reference genome, three PacBio phased diploid genomes and whole-genome resequencing data from multiple genotypes. Two genes, VviTPS04 and -10, were identified as putative paralogues and/or allelic variants. Subsequent gene isolation from multiple grapevine genotypes and characterisation by means of a heterologous in planta expression and volatile analysis resulted in the identification of genotype-specific structural variations and polymorphisms that impact the gene function. These results present novel insight into how grapevine domestication likely shaped the VviTPS landscape to result in genotype-specific functions.en_ZA
dc.description.urihttps://www.mdpi.com/2223-7747/10/8/1520
dc.description.versionPublisher's versionen_ZA
dc.format.extent18 pages : illustrationsen_ZA
dc.identifier.citationSmit, S. J., Vivier, M. A. & Young, P. R. 2021. Seeing the forest through the (phylogenetic) trees : functional characterisation of grapevine terpene synthase (VviTPS) paralogues and orthologues. Plants, 10(8):1520, doi:10.3390/plants10081520.en_ZA
dc.identifier.issn2223-7747 (online)
dc.identifier.otherdoi:10.3390/plants10081520
dc.identifier.urihttp://hdl.handle.net/10019.1/124022
dc.language.isoen_ZAen_ZA
dc.publisherMDPI
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectGrapevinesen_ZA
dc.subjectEcophysiological functionsen_ZA
dc.subjectBiochemical functionsen_ZA
dc.subjectGrapevine terpene synthaseen_ZA
dc.subjectSesquiterpenesen_ZA
dc.subjectWine -- Flavor and odoren_ZA
dc.titleSeeing the forest through the (phylogenetic) trees : functional characterisation of grapevine terpene synthase (VviTPS) paralogues and orthologuesen_ZA
dc.typeArticleen_ZA
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