Purification of sputum samples through sucrose improves detection of mycobacterium tuberculosis by polymerase chain reaction

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dc.contributor.authorVictor T.
dc.contributor.authorDu Toit R.
dc.contributor.authorVan Helden P.D.
dc.date.accessioned2011-05-15T15:56:59Z
dc.date.available2011-05-15T15:56:59Z
dc.date.issued1992
dc.description.abstractA method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a desktop centrifuge allow direct amplification of a 123-bp repetitive region of the M. tuberculosis genome. We have evaluated the sensitivity and specificity of the sucrose method with 155 sputum specimens from patients suspected of having tuberculosis and from normal healthy volunteers. This method, in our opinion, is reproducible, sensitive, and reliable. Multiple samples can be handled simultaneously, and results can be obtained in one working day.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation30
dc.identifier.citation6
dc.identifier.issn951137
dc.identifier.urihttp://hdl.handle.net/10019.1/10145
dc.subjectsodium hydroxide
dc.subjectarticle
dc.subjectbacterium detection
dc.subjectcontrolled study
dc.subjectdiagnostic accuracy
dc.subjecthuman
dc.subjectmajor clinical study
dc.subjectmycobacterium tuberculosis
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectpurification
dc.subjectsputum analysis
dc.subjectCentrifugation, Density Gradient
dc.subjectDNA, Bacterial
dc.subjectHuman
dc.subjectMycobacterium tuberculosis
dc.subjectPolymerase Chain Reaction
dc.subjectSensitivity and Specificity
dc.subjectSputum
dc.subjectSucrose
dc.subjectTuberculosis
dc.titlePurification of sputum samples through sucrose improves detection of mycobacterium tuberculosis by polymerase chain reaction
dc.typeArticle
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