Plasmid Evolution and Interaction between the Plasmid Addiction Stability Systems of Two Related Broad-Host-Range IncQ-Like Plasmids

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dc.contributor.authorDeane S.M.
dc.contributor.authorRawlings D.E.
dc.date.accessioned2011-05-15T16:01:52Z
dc.date.available2011-05-15T16:01:52Z
dc.date.issued2004
dc.description.abstractPlasmid pTC-F14 contains a plasmid stability system called pas (plasmid addiction system), which consists of two proteins, a PasA antitoxin and a PasB toxin. This system is closely related to the pas of plasmid pTF-FC2 (81 and 72% amino acid identity for PasA and PasB, respectively) except that the pas of pTF-FC2 contains a third protein, PasC. As both pTC-F14 and pTF-FC2 are highly promiscuous broad-host-range plasmids isolated from bacteria that share a similar ecological niche, the plasmids are likely to encounter each other. We investigated the relative efficiencies of the two stability systems and whether they had evolved apart sufficiently for each pas to stabilize a plasmid in the presence of the other. The three-component pTF-FC2 pas was more efficient at stabilization of a heterologous tester plasmid than the two component pas of pTC-F14 in Escherichia coli host cells (± 92% and ± 60% after 100 generations, respectively). The PasA antidote of each pas was unable to neutralize the PasB toxin of the other plasmid. The pas proteins of each plasmid autoregulated their own expression as well as that of the pas of the other plasmid. The pas of pTF-FC2 was more effective at repressing the pas operon of pTC-F14 than the pas of pTC-F14 was able to repress itself or the pas of pTF-FC2. This increased efficiency was not due to the PasC of pTF-FC2. The effect of this stronger repression was that pTF-FC2 displaced pTC-F14 when the two plasmids were coresident in the same E. coli host cell. Plasmid curing resulted in the arrest of cell growth but did not cause cell death, and plasmid stability was not influenced by the E. coli mazEF genes.
dc.description.versionArticle
dc.identifier.citationJournal of Bacteriology
dc.identifier.citation186
dc.identifier.citation7
dc.identifier.issn219193
dc.identifier.other10.1128/JB.186.7.2123-2133.2004
dc.identifier.urihttp://hdl.handle.net/10019.1/12196
dc.subjectamino acid
dc.subjectantitoxin
dc.subjectbacterial toxin
dc.subjectPasA antitoxin
dc.subjectPasB toxin
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectautoregulation
dc.subjectbacterial gene
dc.subjectcell death
dc.subjectcell growth
dc.subjectEscherichia coli
dc.subjectevolution
dc.subjectgene expression regulation
dc.subjectgene interaction
dc.subjectgene repression
dc.subjectgenetic stability
dc.subjecthost range
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectplasmid
dc.subjectplasmid addiction system
dc.subjectpriority journal
dc.subjectAntidotes
dc.subjectBacterial Proteins
dc.subjectBacterial Toxins
dc.subjectBase Sequence
dc.subjectConjugation, Genetic
dc.subjectDNA Helicases
dc.subjectDNA-Binding Proteins
dc.subjectEscherichia coli
dc.subjectEvolution, Molecular
dc.subjectGene Expression Regulation, Bacterial
dc.subjectMolecular Sequence Data
dc.subjectOperon
dc.subjectPlasmids
dc.subjectProteins
dc.subjectSequence Analysis, DNA
dc.subjectTrans-Activators
dc.subjectBacteria (microorganisms)
dc.subjectbroad host range plasmids
dc.subjectEscherichia coli
dc.subjectNegibacteria
dc.titlePlasmid Evolution and Interaction between the Plasmid Addiction Stability Systems of Two Related Broad-Host-Range IncQ-Like Plasmids
dc.typeArticle
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