The Acridine Orange test: Determining the relationship between sperm morphology and fertilization in vitro

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dc.contributor.authorClaassens O.E.
dc.contributor.authorMenkveld S.
dc.contributor.authorFranken D.R.
dc.contributor.authorPretorius E.
dc.contributor.authorSwart Y.
dc.contributor.authorLombard C.J.
dc.contributor.authorKruger T.F.
dc.date.accessioned2011-05-15T16:16:35Z
dc.date.available2011-05-15T16:16:35Z
dc.date.issued1992
dc.description.abstractThe prediction of human fertilization is an important aspect of research protocols dealing with male fertility. Sperm DNA has been reported to be an indicator of human sperm fertility potential. The Acridine Orange test for evaluation of sperm DNA has been employed during the present study to determine its relationship with human sperm morphology and fertilization in vitro. Seventy-six patients from the in-vitro fertilization (IVF) and/or gamete intra-Fallopian transfer (GIFT) programme were randomly selected for the study. All patients had a routine semen analysis, sperm DNA evaluation and underwent standard IVF procedures at the time of the study. The results indicated a moderate positive correlation (r=0.38, P=0.0006) between results of the Acridine Orange test and normal sperm morphology. Patients with an Acridine Orange test value exceeding 24% had significantly higher oocyte fertilization rates than patients with lower values, for metaphase I (74 versus 51%, P=0.0008) and for metaphase II oocytes (88 versus 60%, P=0.0001). Sperm morphology, however, proved to be a more significant predictor of fertilization in vitro compared to the Acridine Orange test.
dc.description.versionArticle
dc.identifier.citationHuman Reproduction
dc.identifier.citation7
dc.identifier.citation2
dc.identifier.issn02681161
dc.identifier.urihttp://hdl.handle.net/10019.1/13849
dc.subjectacridine orange
dc.subjectarticle
dc.subjectfemale
dc.subjectfertilization in vitro
dc.subjectgamete intrafallopian transfer
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectmorphology
dc.subjectoocyte
dc.subjectsemen analysis
dc.subjectsperm
dc.subjectspermatozoon density
dc.subjectspermatozoon motility
dc.subjectAcridine Orange
dc.subjectComparative Study
dc.subjectFertilization in Vitro
dc.subjectHuman
dc.subjectIn Vitro
dc.subjectMale
dc.subjectSperm Count
dc.subjectSperm Motility
dc.subjectSpermatozoa
dc.titleThe Acridine Orange test: Determining the relationship between sperm morphology and fertilization in vitro
dc.typeArticle
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