Localization of tyrosine phosphorylated proteins in human sperm and relation to capacitation and zona pellucida binding

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dc.contributor.authorSakkas D.
dc.contributor.authorLeppens-Luisier G.
dc.contributor.authorLucas H.
dc.contributor.authorChardonnens D.
dc.contributor.authorCampana A.
dc.contributor.authorFranken D.R.
dc.contributor.authorUrner F.
dc.date.accessioned2011-05-15T16:15:41Z
dc.date.available2011-05-15T16:15:41Z
dc.date.issued2003
dc.description.abstractMammalian sperm must undergo a process known as capacitation before fertilization can take place. A key intracellular event that occurs during capacitation is protein tyrosine phosphorylation. The objective of this study was to investigate and visualize protein tyrosine phosphorylation patterns in human sperm during capacitation and interaction with the zona pellucida. The presence of specific patterns was also assessed in relation to the fertilizing capacity of the spermatozoa after in vitro fertilization. Protein tyrosine phosphorylation was investigated by immunofluorescence. Phosphorylation increased significantly with capacitation and was localized mainly to the principal piece of human sperm. Following binding to the zona pellucida, the percentage of sperm with phosphotyrosine residues localized to both the neck and the principal piece was significantly higher in bound sperm than in capacitated sperm in suspension. When the percentage of principal piece-positive sperm present after capacitation was <7%, fertilization rates after in vitro fertilization were reduced. Different compartments of human spermatozoa undergo a specific sequence of phosphorylation during both capacitation and upon binding to the zona pellucida. Tyrosine phosphorylation in the principal and neck piece may be considered a prerequisite for fertilization in humans.
dc.description.versionArticle
dc.identifier.citationBiology of Reproduction
dc.identifier.citation68
dc.identifier.citation4
dc.identifier.issn00063363
dc.identifier.other10.1095/biolreprod.102.011023
dc.identifier.urihttp://hdl.handle.net/10019.1/13443
dc.subjectphosphotyrosine
dc.subjectprotein
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectfertilization
dc.subjectfertilization in vitro
dc.subjectgamete
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunofluorescence
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmammal
dc.subjectpriority journal
dc.subjectprotein binding
dc.subjectprotein interaction
dc.subjectprotein localization
dc.subjectprotein phosphorylation
dc.subjectsperm
dc.subjectspermatozoon capacitation
dc.subjectzona pellucida
dc.subjectFemale
dc.subjectFertilization
dc.subjectHumans
dc.subjectMale
dc.subjectPhosphotyrosine
dc.subjectProteins
dc.subjectSperm Capacitation
dc.subjectSpermatozoa
dc.subjectTissue Distribution
dc.subjectTyrosine
dc.subjectZona Pellucida
dc.subjectFallopia
dc.subjectMammalia
dc.titleLocalization of tyrosine phosphorylated proteins in human sperm and relation to capacitation and zona pellucida binding
dc.typeArticle
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