Growth optimization of pediococcus damnosus NCFB 1832 and the influence of pH and nutrients on the production of pediocin PD-1

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dc.contributor.authorNel H.A.
dc.contributor.authorBauer R.
dc.contributor.authorVandamme E.J.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:59:25Z
dc.date.available2011-05-15T15:59:25Z
dc.date.issued2001
dc.description.abstractAims: Optimization of the growth of Pediococcus damnosus NCFB 1832 and the production of pediocin PD-1 by traditional fermentation methods. Methods and Results: Fermentation studies were conducted in De Man Rogosa and Sharpe (MRS) broth (Oxoid), preadjusted to specific pH values, and in MRS broth supplemented with various nitrogen sources, MnSO4, MgSO4 and Tween 80. The production of pediocin PD-1 closely followed the growth curve of Ped. damnosus NCFB 1832. Maximum levels of bacteriocin activity (3249 AU ml-1/O.D.max) were recorded in MRS broth with an initial pH of 6.7. In media with an initial pH of 4.5 bacteriocin activity as low as 222 AU ml-1/O.D.max was recorded. The highest bacteriocin activity was recorded in growth conditions allowing the greatest pH variation (highest ΔpH). The addition of bacteriological peptone (1.7%, w/v), MnSO4 (0.014%, w/v) and Tween 80 (3%, v/v) to MRS and adjustment of the medium pH to 6.7 resulted in a further increase in activity (from 3249 to 5078 AU ml-1/O.D.max). The same medium, but with an initial pH of 6.2, resulted in an 82.5% decrease in bacteriocin activity. Conclusions: Pediocin PD-1 production is not only stimulated by the presence of specific growth factors (e.g. bacteriological peptone, MnSO4 or Tween 80), but may also be stimulated by the lowering in pH during growth (highest ΔpH), and thus also the amount of organic acids produced. Significance and Impact of the Study: The production of pediocin PD-1 by the wild-type producer strain was significantly improved by using a defined medium and traditional fermentation methods.
dc.description.versionArticle
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation91
dc.identifier.citation6
dc.identifier.issn13645072
dc.identifier.other10.1046/j.1365-2672.2001.01486.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11164
dc.subjectbacteriocin
dc.subjectcarboxylic acid
dc.subjectgrowth factor
dc.subjectmagnesium sulfate
dc.subjectmanganese sulfate
dc.subjectnitrogen
dc.subjectpediocin
dc.subjectpeptone
dc.subjectpolysorbate 80
dc.subjectbacterium
dc.subjectgrowth
dc.subjectnutrient
dc.subjectpH
dc.subjectarticle
dc.subjectbacterial growth
dc.subjectcontrolled study
dc.subjectfermentation
dc.subjectgrowth curve
dc.subjectnonhuman
dc.subjectnutrient
dc.subjectPediococcus
dc.subjectPediococcus damnosus
dc.subjectpH
dc.subjectprotein analysis
dc.subjectprotein synthesis
dc.subjectAnti-Bacterial Agents
dc.subjectBacteriocins
dc.subjectBiotechnology
dc.subjectCulture Media
dc.subjectFermentation
dc.subjectHydrogen-Ion Concentration
dc.subjectPediococcus
dc.subjectPeptides
dc.titleGrowth optimization of pediococcus damnosus NCFB 1832 and the influence of pH and nutrients on the production of pediocin PD-1
dc.typeArticle
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