Intra- and inter-laboratory variability in the assessment of sperm morphology by strict criteria: Impact of semen preparation, staining techniques and manual versus computerized analysis

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dc.contributor.authorBarroso G.
dc.contributor.authorMercan R.
dc.contributor.authorOzgur K.
dc.contributor.authorMorshedi M.
dc.contributor.authorKolm P.
dc.contributor.authorCoetzee K.
dc.contributor.authorKruger T.
dc.contributor.authorOehninger S.
dc.date.accessioned2011-05-15T16:16:34Z
dc.date.available2011-05-15T16:16:34Z
dc.date.issued1999
dc.description.abstractWe designed prospective studies to compare manual and computerized analysis of sperm morphology by strict criteria using different semen processing and staining techniques. A total of 54 semen samples were studied; slides were prepared from each subject from liquefied semen and after washing, and stained with Diff-Quik® or Papanicolaou. An intra-laboratory, blind assessment was performed manually (two observers) and using a computerized analyser (two readings). This demonstrated a very good correlation between manual analysis of liquefied and washed samples with both staining techniques [intraclass coefficient (ICC) = 0.93 and 0.83]. Greater agreement was observed between computerized readings (washed samples) of Diff-Quik® (ICC = 0.93) than of Papanicolaou-stained slides (ICC = 0.66). An excellent intra-laboratory correlation was observed for within-computer readings (ICC = 0.93). There was moderate agreement between inter-laboratory computer readings (two centres, ICC = 0.72). Although there was lower inter-laboratory agreement for manual and manual versus computer readings, overall results of all manual and computer analyses showed good agreement (ICC = 0.73). Diff-Quik® staining is reliable for both manual (liquefied) and computer (washed) analysis of strict sperm morphology. Intra- and inter-computer analyses using this method reached satisfactory levels of agreement. There is still high inter-laboratory variability for the manual method.
dc.description.versionArticle
dc.identifier.citationHuman Reproduction
dc.identifier.citation14
dc.identifier.citation8
dc.identifier.issn02681161
dc.identifier.other10.1093/humrep/14.8.2036
dc.identifier.urihttp://hdl.handle.net/10019.1/13840
dc.subjectarticle
dc.subjectcomputer analysis
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectmorphology
dc.subjectprospective study
dc.subjectseminal plasma
dc.subjectspermatozoon
dc.subjectstaining
dc.subjectAdult
dc.subjectCell Separation
dc.subjectHumans
dc.subjectImage Processing, Computer-Assisted
dc.subjectMale
dc.subjectProspective Studies
dc.subjectReference Standards
dc.subjectSpermatozoa
dc.subjectStaining and Labeling
dc.titleIntra- and inter-laboratory variability in the assessment of sperm morphology by strict criteria: Impact of semen preparation, staining techniques and manual versus computerized analysis
dc.typeArticle
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