A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 represser gene

Simple item page
dc.contributor.authorLambrechts M.G.
dc.contributor.authorSollitti P.
dc.contributor.authorMurmur J.
dc.contributor.authorPretorius I.S.
dc.date.accessioned2011-05-15T16:01:29Z
dc.date.available2011-05-15T16:01:29Z
dc.date.issued1996
dc.description.abstractTranscription of the three unlinked, homologous STA1-3 glucoamylase-encoding genes, involved in starch degradation by Saccharomyces cerevisiae, was previously shown to be down-regulated by the presence of STA10, acting via three upstream repression sequence regions that were identified in the STA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene, MSS10 (multicopy suppressor of STA10), which, when present in multiple copies, overcomes STA10 repression. Deletion of MSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence of MSS10 is identical to three other genes from S. cerevisiae identified as: FUP1, a gene that enhances iron-limited growth; PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; and MSN1, a gene encoding a transcriptional activator involved in invertase regulation.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation29
dc.identifier.citation6
dc.identifier.issn1728083
dc.identifier.urihttp://hdl.handle.net/10019.1/12004
dc.subjectfungal protein
dc.subjectglucan 1,4 alpha glucosidase
dc.subjectstarch
dc.subjecttranscription factor
dc.subjectfungal DNA
dc.subjectarticle
dc.subjectcarbohydrate metabolism
dc.subjectcontrolled study
dc.subjectenzyme synthesis
dc.subjectfungal genetics
dc.subjectgene deletion
dc.subjectgene number
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectrepressor gene
dc.subjectsaccharomyces cerevisiae
dc.subjectsequence homology
dc.subjectsuppressor gene
dc.subjecttranscription regulation
dc.subjectbiosynthesis
dc.subjectdiploidy
dc.subjectenzymology
dc.subjectfungal gene
dc.subjectgene expression
dc.subjectgenetics
dc.subjectheterozygote
dc.subjectmetabolism
dc.subjectmultigene family
dc.subjectregulator gene
dc.subjectrestriction mapping
dc.subjectSaccharomyces cerevisiae
dc.subjecttransactivation
dc.subjectCloning, Molecular
dc.subjectDiploidy
dc.subjectDNA, Fungal
dc.subjectGene Expression
dc.subjectGenes, Fungal
dc.subjectGenes, Regulator
dc.subjectGenes, Suppressor
dc.subjectGlucan 1,4-alpha-Glucosidase
dc.subjectHeterozygote
dc.subjectMultigene Family
dc.subjectRestriction Mapping
dc.subjectSaccharomyces cerevisiae
dc.subjectStarch
dc.subjectTrans-Activation (Genetics)
dc.titleA multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 represser gene
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications