Oligonucleotide (GTG)5 as a marker for Mycobacterium tuberculosis strain identification

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dc.contributor.authorWiid J.J.F.
dc.contributor.authorWerely C.
dc.contributor.authorBeyers N.
dc.contributor.authorDonald P.
dc.contributor.authorVan Helden P.D.
dc.date.accessioned2011-05-15T15:56:58Z
dc.date.available2011-05-15T15:56:58Z
dc.date.issued1994
dc.description.abstractCulture of Mycobacterium tuberculosis provides no information on the identity of a strain or the distribution of such a strain in the community. Strain identification of M. tuberculosis can help to address important epidemiological questions, e.g., the origin of an infection in a patient's household or community, whether reactivation of infection is endogenous or exogenous in origin, and the spread and early detection of organisms with acquired antibiotic resistance. To research this problem, strain identification must be reliable and accurate. Although genetic identification techniques already exist, it is valuable to have genetic identification techniques based on a number of genetic markers to improve the accurate identification of M. tuberculosis strains. We show that oligonucleotide (GTG)5 can be successfully applied to the identification of M. tuberculosis strains. This technique may be particularly useful in cases in which M. tuberculosis strains have few or no insertion elements (e.g., IS6110) or in identifying other strains of mycobacteria when informative probes are lacking.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation32
dc.identifier.citation5
dc.identifier.issn951137
dc.identifier.urihttp://hdl.handle.net/10019.1/10144
dc.subjectdna marker
dc.subjectoligonucleotide
dc.subjectarticle
dc.subjectbacterium detection
dc.subjectbacterium identification
dc.subjectgenetic marker
dc.subjectmycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectstrain difference
dc.subjectAdult
dc.subjectBacterial Typing Techniques
dc.subjectBase Sequence
dc.subjectChild
dc.subjectDNA Fingerprinting
dc.subjectDNA Probes
dc.subjectDNA Transposable Elements
dc.subjectDNA, Bacterial
dc.subjectEvaluation Studies
dc.subjectFemale
dc.subjectGenetic Markers
dc.subjectHuman
dc.subjectMale
dc.subjectMiddle Age
dc.subjectMolecular Sequence Data
dc.subjectMycobacterium tuberculosis
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectRepetitive Sequences, Nucleic Acid
dc.subjectSouth Africa
dc.subjectSpecies Specificity
dc.subjectSupport, Non-U.S. Gov't
dc.subjectTuberculosis, Pulmonary
dc.titleOligonucleotide (GTG)5 as a marker for Mycobacterium tuberculosis strain identification
dc.typeArticle
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