Proteomic analysis of mitochondrial proteins in a mouse model of type 2 diabetes

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dc.contributor.authorEssop M.F.
dc.contributor.authorChan W.A.
dc.contributor.authorHattingh S.
dc.date.accessioned2011-10-13T16:59:17Z
dc.date.available2011-10-13T16:59:17Z
dc.date.issued2011
dc.description.abstractObjective: Impaired mitochondrial function may contribute to the onset of contractile dysfunction with insulin resistance/ type 2 diabetes. Our aim was therefore to determine alterations in the mitochondrial proteome of a mouse model of obesity/type 2 diabetes. Methods: Mitochondrial proteins were isolated from hearts collected from 18- to 20-week-old female db/db mice and compared to matched controls. We performed two-dimensional polyacrylamide gel electrophoresis to determine differentially expressed proteins. Peptides of interest were further analysed by mass spectrometry and Mascot software was employed to identify protein matches. Results: Our data showed that ATP synthase D chain, ubiquinol cytochrome-C reductase core protein 1 and electron transfer flavoprotein subunit alpha peptide levels were altered with obesity. Moreover, we found coordinate downregulation of contractile proteins in the obese heart, i.e. α-smooth muscle actin, α-cardiac actin, myosin heavy-chain α and myosin-binding protein C. Conclusion: We propose that decreased contractile protein levels may contribute to contractile dysfunction of hearts from diabetic mice.
dc.description.versionArticle
dc.identifier.citationCardiovascular Journal of Africa
dc.identifier.citation22
dc.identifier.citation4
dc.identifier.citationhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80052223023&partnerID=40&md5=3a0f99a2f20486a8adbdadcf50f0b322
dc.identifier.issn19951892
dc.identifier.other10.5830/CVJA-2010-058
dc.identifier.urihttp://hdl.handle.net/10019.1/17054
dc.titleProteomic analysis of mitochondrial proteins in a mouse model of type 2 diabetes
dc.typeArticle
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