Molecular analysis of the malic enzyme gene (mae2) of Schizosaccharomyces pombe

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dc.contributor.authorViljoen M.
dc.contributor.authorSubden R.E.
dc.contributor.authorKrizus A.
dc.contributor.authorVan Vuuren H.J.J.
dc.date.accessioned2011-05-15T16:05:35Z
dc.date.available2011-05-15T16:05:35Z
dc.date.issued1994
dc.description.abstractSequence analysis of a 4.6-kb HindIII fragment containing the malic enzyme gene (mae2) of Schizosaccharomyces pombe, revealed the presence of an open reading frame of 1695 nucleotides, coding for a 565 amino acid polypeptide. The mae2 gene is expressed constitutively and encodes a single mRNA transcript of 2.0 kb. The mae2 gene was mapped on chromosome III by chromoblotting. The coding region and inferred amino acid sequence showed significant homology with 12 malic enzyme genes and proteins from widely different origins. Eight highly homologous regions were found in these malic enzymes, suggesting that they contain functionally conserved amino acid sequences that are indispensable for activity of malic enzymes. Two of these regions have previously been reported to be NAD- and NADP-binding sites.
dc.description.versionArticle
dc.identifier.citationYeast
dc.identifier.citation10
dc.identifier.citation5
dc.identifier.issn0749503X
dc.identifier.other10.1002/yea.320100506
dc.identifier.urihttp://hdl.handle.net/10019.1/13201
dc.subjectmalate dehydrogenase (decarboxylating)
dc.subjectarticle
dc.subjectchromosome 3
dc.subjectdna sequence
dc.subjectgene mapping
dc.subjectgene structure
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectschizosaccharomyces pombe
dc.subjectsequence homology
dc.subjectwine
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectBlotting, Northern
dc.subjectChromosome Mapping
dc.subjectDNA Probes
dc.subjectGenes, Fungal
dc.subjectMalate Dehydrogenase
dc.subjectMolecular Sequence Data
dc.subjectNAD
dc.subjectNADP
dc.subjectSchizosaccharomyces
dc.subjectSequence Analysis, DNA
dc.subjectSequence Homology, Amino Acid
dc.subjectSupport, Non-U.S. Gov't
dc.titleMolecular analysis of the malic enzyme gene (mae2) of Schizosaccharomyces pombe
dc.typeArticle
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