Effects of pH and ionic strength on the binding of L-tri-iodothyronine to the solubilized nuclear receptor

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dc.contributor.authorWilson B.D.
dc.contributor.authorGent W.L.
dc.date.accessioned2011-05-15T16:03:25Z
dc.date.available2011-05-15T16:03:25Z
dc.date.issued1985
dc.description.abstractK'(A) (apparent association constant) and B(max.) (total receptor concentration) describing the interaction of tri-iodothyronine (T3) and it solubulized rat liver nuclear receptor (R) are found to be moderately consistent in successive preparations, but both quantities diminished after a few days. To achieve comparability in the effects of ionic strenght (I) and of pH on K'(A) and B(max.) appropriate measurements have been made simultaneously on single preparations. K'(A) and B(max.) were found to be effectively unchanged over the range I 0.05-0.60. Both parameters have been measured over the range pH 6.4-9.0 and the vlaues of K'(A) analysed in terms of the 4'-OH ionization of T3 and that of a cationic acidic group, shown to require pK' = 7.6. This group could be identified either with the terminal α-NH3+ of T3 or with a group (RH+) in the receptor site. On the balance of evidence the first possibility is more likely, in which case the variation of B(max.) with pH is ascribed to conformational changes in the receptor protein.
dc.description.versionArticle
dc.identifier.citationBiochemical Journal
dc.identifier.citation232
dc.identifier.citation3
dc.identifier.issn2646021
dc.identifier.urihttp://hdl.handle.net/10019.1/12611
dc.subjectcell nucleus receptor
dc.subjecthormone receptor
dc.subjectliothyronine
dc.subjectliothyronine i 125
dc.subjectliothyronine receptor
dc.subjectradioisotope
dc.subjectanimal cell
dc.subjectcell nucleus
dc.subjectchemical bond
dc.subjectdrug receptor binding
dc.subjectliver
dc.subjectnonhuman
dc.subjectph
dc.subjectpharmacokinetics
dc.subjectpriority journal
dc.subjectrat
dc.subjectAnimal
dc.subjectBinding Sites
dc.subjectCell Nucleus
dc.subjectHydrogen-Ion Concentration
dc.subjectKinetics
dc.subjectLiver
dc.subjectOsmolar Concentration
dc.subjectRats
dc.subjectReceptors, Cell Surface
dc.subjectReceptors, Thyroid Hormone
dc.subjectTriiodothyronine
dc.titleEffects of pH and ionic strength on the binding of L-tri-iodothyronine to the solubilized nuclear receptor
dc.typeArticle
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