Enhancing effect of temperature on the transmucosal penetration kinetics of 17 beta-estradiol.
| dc.contributor.author | van der Bijl P. | |
| dc.contributor.author | van Eyk A.D. | |
| dc.contributor.author | van der Bijl P. | |
| dc.contributor.author | Kriel J. | |
| dc.date.accessioned | 2011-05-15T15:54:12Z | |
| dc.date.available | 2011-05-15T15:54:12Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | Human vaginal mucosa may be used as a model of buccal mucosa for in vitro permeability studies using various chemical compounds. Rectilinear temperature dependence of water flux across vaginal mucosa between 25 degrees C and 41 degrees C has been shown. The objective of this study was to examine the behaviour of the above barrier on fluxes of 17 beta-estradiol at various temperatures. Frozen vaginal mucosa specimens from a single patient were used (snap-frozen in liquid nitrogen and stored at -85 degrees C). The permeability to tritiated 17 beta-estradiol was determined using a continous flow-through perfusion system at temperatures of 20 degrees C, 25 degrees C, 30 degrees C, 37 degrees C and 41 degrees C. Histological examinations were performed before and after permeability experiments. Estimated steady state flux values were used at 20 degrees C, 25 degrees C and 30 degrees C. Estimated and true mean 17 beta-estradiol steady state flux rates (20-24 h) were found to be 415 +/- 27 standard error of the Mean (SEM), 848 +/- 60 SEM, 1355 +/- 77 SEM, 1436 +/- 37 SEM and 1482 +/- 35 SEM cpm.cm-2.min-1, at temperatures of 20 degrees C, 25 degrees C, 30 degrees C, 37 degrees C and 41 degrees C, respectively. A non-linear regression analysis and plot (R2 = 0.9941) displayed a curvilinear flux-temperature relationship. The results from this study showed that, notwithstanding cellular damage, the principal physical permeability barrier governing permeation kinetics was non-linearly temperature-dependent between the temperatures studied, providing further support for the concept that this barrier is lipoidal in nature. | |
| dc.description.version | Article | |
| dc.identifier.citation | SADJ : journal of the South African Dental Association = tydskrif van die Suid-Afrikaanse Tandheelkundige Vereniging | |
| dc.identifier.citation | 58 | |
| dc.identifier.citation | 3 | |
| dc.identifier.issn | 10294864 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/9057 | |
| dc.title | Enhancing effect of temperature on the transmucosal penetration kinetics of 17 beta-estradiol. | |
| dc.type | Article |