Genomic characterization of an endemic Mycobacterium tuberculosis strain: Evolutionary and epidemiologic implications

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dc.contributor.authorNguyen D.
dc.contributor.authorBrassard P.
dc.contributor.authorMenzies D.
dc.contributor.authorThibert L.
dc.contributor.authorWarren R.
dc.contributor.authorMostowy S.
dc.contributor.authorBehr M.
dc.date.accessioned2011-05-15T15:56:56Z
dc.date.available2011-05-15T15:56:56Z
dc.date.issued2004
dc.description.abstractIn a study of 302 Mycobacterium tuberculosis clinical isolates from the low-incidence Canadian-born population of Quebec, we characterized a large endemic strain family by using genomic deletions. The DS6Quebec deleted region (11.4 kb) defined a strain family of 143 isolates encompassing two subgroups: one characterized by pyrazinamide (PZA) susceptibility and the other marked by a PZA-monoresistant phenotype. A second deletion (8 bp) in the pncA gene was shared by all 76 isolates with the PZA resistance phenotype, whereas a third DRv0961 deletion (970 bp) defined a further subset of 15 isolates. From their deletion profiles, we derived a most parsimonious evolutionary scenario and compared multiple standard genotyping modalities (using IS6110 restriction fragment length polymorphism [RFLP], spoligotyping, and mycobacterial interspersed repetitive units [MIRU]) across the deletion-based subgroups. The use of a single genotyping modality yielded an unexpectedly high proportion of clustered isolates for a high IS6110 copy strain (27% by IS6110 RFLP, 61% by MIRU, and 77% by spoligotyping). By combining all three modalities, only 14% were genotypically clustered overall, a result more congruent with the epidemiologic profile of reactivation tuberculosis, as suggested by the older age (mean age, 60 years), rural setting, and low proportion of epidemiologic links. These results provide insight into the evolution of genotypes in endemic strains and the potential for false clustering in molecular epidemiologic studies.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation42
dc.identifier.citation6
dc.identifier.issn951137
dc.identifier.other10.1128/JCM.42.6.2573-2580.2004
dc.identifier.urihttp://hdl.handle.net/10019.1/10124
dc.subjectgenomic DNA
dc.subjectpyrazinamide
dc.subjecttuberculostatic agent
dc.subjectadult
dc.subjectantibiotic sensitivity
dc.subjectarticle
dc.subjectbacterial genome
dc.subjectCanada
dc.subjectendemic disease
dc.subjectfemale
dc.subjectgene
dc.subjectgene mutation
dc.subjectgenotype
dc.subjecthuman
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectminimum inhibitory concentration
dc.subjectMycobacterium
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectphenotype
dc.subjectpnca gene
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectrestriction fragment length polymorphism
dc.subjectstrain difference
dc.subjecttuberculosis
dc.subjectEvolution
dc.subjectGenome, Bacterial
dc.subjectGenotype
dc.subjectMycobacterium tuberculosis
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectRepetitive Sequences, Nucleic Acid
dc.subjectActinobacteria (class)
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectMycobacterium
dc.subjectMycobacterium tuberculosis
dc.subjectProkaryota
dc.subjectuncultured actinomycete
dc.titleGenomic characterization of an endemic Mycobacterium tuberculosis strain: Evolutionary and epidemiologic implications
dc.typeArticle
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