Degradation of aflatoxin B1 by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556T

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dc.contributor.authorTeniola O.D.
dc.contributor.authorAddo P.A.
dc.contributor.authorBrost I.M.
dc.contributor.authorFarber P.
dc.contributor.authorJany K.-D.
dc.contributor.authorAlberts J.F.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorSteyn P.S.
dc.contributor.authorHolzapfel W.H.
dc.date.accessioned2011-05-15T15:56:48Z
dc.date.available2011-05-15T15:56:48Z
dc.date.issued2005
dc.description.abstractBiological degradation of aflatoxin B1 (AFB1) by Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB1 was observed during incubation in the presence of R. erythropolis cells (17% residual AFB1 after 48 h and only 3-6% residual AFB1 after 72 h). Cell-free extracts of four bacterial strains, R. erythropolis DSM 14303, Nocardia corynebacterioides DSM 12676, N. corynebacterioides DSM 20151, and Mycobacterium fluoranthenivorans sp. nov. DSM 44556T were produced by disrupting cells in a French pressure cell. The ability of crude cell-free extracts to degrade AFB 1 was studied under different incubation conditions. Aflatoxin B 1 was effectively degraded by cell free extracts of all four bacterial strains. N. corynebacterioides DSM 12676 (formerly erroneously classified as Flavobacterium aurantiacum) showed the lowest degradation ability (60%) after 24 h, while >90% degradation was observed with N. corynebacterioides DSM 20151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44556T have shown more than 90% degradation of AFB1 within 4 h at 30°C, whilst after 8 h AFB 1 was practicably not detectable. The high degradation rate and wide temperature range for degradation by R. erythropolis DSM 14303 and M. fluoranthenivorans sp. nov. DSM 44556T indicate potential for application in food and feed processing. © 2005 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Food Microbiology
dc.identifier.citation105
dc.identifier.citation2
dc.identifier.issn1681605
dc.identifier.other10.1016/j.ijfoodmicro.2005.05.004
dc.identifier.urihttp://hdl.handle.net/10019.1/10046
dc.subjectaflatoxin B1
dc.subjectanimal cell
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectbacterium detection
dc.subjectcell disruption
dc.subjectcell free system
dc.subjectfood processing
dc.subjectincubation time
dc.subjectMycobacterium
dc.subjectMycobacterium fluoranthenivoran
dc.subjectnonhuman
dc.subjectpressure chamber
dc.subjectprotein degradation
dc.subjectreduction
dc.subjectRhodococcus erythropolis
dc.subjecttemperature measurement
dc.subjectAflatoxin B1
dc.subjectBiodegradation, Environmental
dc.subjectMycobacterium
dc.subjectNocardia
dc.subjectRhodococcus
dc.subjectTemperature
dc.subjectTime Factors
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectFlavobacterium aurantiacum
dc.subjectMycobacterium
dc.subjectMycobacterium fluoranthenivorans
dc.subjectNocardia corynebacterioides
dc.subjectRhodococcus erythropolis
dc.titleDegradation of aflatoxin B1 by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556T
dc.typeArticle
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