Chromosomal evolution in Rattini (Muridae, Rodentia)

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dc.contributor.authorBadenhorst D.
dc.contributor.authorDobigny G.
dc.contributor.authorAdega F.
dc.contributor.authorChaves R.
dc.contributor.authorO'Brien P.C.M.
dc.contributor.authorFerguson-Smith M.A.
dc.contributor.authorWaters P.D.
dc.contributor.authorRobinson T.J.
dc.date.accessioned2011-10-13T16:58:28Z
dc.date.available2011-10-13T16:58:28Z
dc.date.issued2011
dc.description.abstractThe Rattini (Muridae, Murinae) includes the biologically important model species Rattus norvegicus (RNO) and represents a group of rodents that are of clinical, agricultural and epidemiological importance. We present a comparative molecular cytogenetic investigation of ten Rattini species representative of the genera Maxomys, Leopoldamys, Niviventer, Berylmys, Bandicota and Rattus using chromosome banding, cross-species painting (Zoo-fluorescent in situ hybridization or FISH) and BAC-FISH mapping. Our results show that these taxa are characterised by slow to moderate rates of chromosome evolution that contrasts with the extensive chromosome restructuring identified in most other murid rodents, particularly the mouse lineage. This extends to genomic features such as NOR location (for example, NORs on RNO 3 are present on the corresponding chromosomes in all species except Bandicota savilei and Niviventer fulvescens, and the NORs on RNO 10 are conserved in all Rattini with the exception of Rattus). The satellite I DNA family detected and characterised herein appears to be taxon (Rattus) specific, and of recent origin (consistent with a feedback model of satellite evolution). BAC-mapping using clones that span regions responsible for the morphological variability exhibited by RNO 1, 12 and 13 (acrocentric/submetacentric) and their orthologues in Rattus species, demonstrated that the differences are most likely due to pericentric inversions as exemplified by data on Rattus tanezumi. Chromosomal characters detected using R. norvegicus and Maxomys surifer whole chromosome painting probes were mapped to a consensus sequence-based phylogenetic tree thus allowing an objective assessment of ancestral states for the reconstruction of the putative Rattini ancestral karyotype. This is thought to have comprised 46 chromosomes that, with the exception of a single pair of metacentric autosomes, were acrocentric in morphology. © 2011 Springer Science+Business Media B.V.
dc.description.versionArticle in Press
dc.identifier.citationChromosome Research
dc.identifier.citationhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80051603734&partnerID=40&md5=0c2cc2c908bc3954cbbe25a744cc8c1b
dc.identifier.issn9673849
dc.identifier.other10.1007/s10577-011-9227-2
dc.identifier.urihttp://hdl.handle.net/10019.1/16736
dc.subjectancestral karyotype
dc.subjectBAC-FISH
dc.subjectChromosome painting
dc.subjectkaryotype evolution
dc.subjectRattini
dc.subjectsatellite DNA
dc.titleChromosomal evolution in Rattini (Muridae, Rodentia)
dc.typeArticle in Press
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