In vitro influence of sublethal hypoxia on differentiation of the 3T3-L1 preadipose cell line and its physiological implications

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dc.contributor.authorMacfarlane C.M.
dc.date.accessioned2011-05-15T16:17:19Z
dc.date.available2011-05-15T16:17:19Z
dc.date.issued1997
dc.description.abstractExposure of cultures of 3T3-L1 preadipose cells to nitrogen for 16 hours kills almost all of the cells, but after exposure to 5% oxygen for 16 hours most of the cells survive, and recover when culture is continued in 20% oxygen. The extent of recovery depends on the insulin concentration of the medium. Isotope incorporation and flow cytometry experiments show that exposure to 5% oxygen for 16 hours growth arrests the cells and leads to an elongation of the G1-phase of the cell cycle. When 3T3-L1 cells are growth arrested in the presence of 5% oxygen and allowed to recover in the presence of 5μg/ml insulin under 20% oxygen, they can be induced to differentiate by treatment with carbacyclin during the period of growth arrest. Activity of the marker enzyme glycerol-3-phosphate dehydrogenase increases from 46.5±17 mU/mg protein to 1506±271 mU/mg protein. The extent of differentiation is exponentially related to the concentration of carbacyclin in the medium.
dc.description.versionArticle
dc.identifier.citationLife Sciences
dc.identifier.citation60
dc.identifier.citation21
dc.identifier.issn00243205
dc.identifier.urihttp://hdl.handle.net/10019.1/14167
dc.subjectadipocyte
dc.subjectanimal
dc.subjectarticle
dc.subjectcell differentiation
dc.subjectcell division
dc.subjectcell hypoxia
dc.subjectcell strain 3T3
dc.subjectcell strain HL 60
dc.subjectculture medium
dc.subjectcytology
dc.subjectelectron microscopy
dc.subjectflow cytometry
dc.subjecthuman
dc.subjectmouse
dc.subjectphysiology
dc.subjectultrastructure
dc.subject3T3 Cells
dc.subjectAdipocytes
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Division
dc.subjectCell Hypoxia
dc.subjectCulture Media
dc.subjectFlow Cytometry
dc.subjectHL-60 Cells
dc.subjectHumans
dc.subjectMice
dc.subjectMicroscopy, Electron
dc.titleIn vitro influence of sublethal hypoxia on differentiation of the 3T3-L1 preadipose cell line and its physiological implications
dc.typeArticle
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