Experimental values of the ionization constants for L-3,5-di-iodotyrosine and a model for ionic interactions of thyroid hormone (T3) and its nuclear receptor

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dc.contributor.authorGent W.L.
dc.contributor.authorWilson B.D.
dc.date.accessioned2011-05-15T16:01:18Z
dc.date.available2011-05-15T16:01:18Z
dc.date.issued1989
dc.description.abstractIonization characteristics of L-3,5-di-iodotyrosine have been measured under various conditions. These data, which correct an erroneous report of pK3 in the literature, have been used to estimate the ionization constants of the thyroid hormone L-3',3,5-tri-iodothyronine (T3). On this basis a reinterpretation has been made of the pH-dependent binding of the hormone to its solubilized rat liver nuclear receptor (Wilson BD and Gent WL, Biochem J 232: 663-667, 1985). The interaction may depend on the ionization of the phenolic group of T3 and an acidic group (pK'7.6) in the receptor site, leading to the formation of a hydrogen bond between the two groups. The changes of the number of binding sites with pH must then result independently from alterations in the conformation of the receptor protein.
dc.description.versionArticle
dc.identifier.citationBiochemical Pharmacology
dc.identifier.citation38
dc.identifier.citation2
dc.identifier.issn62952
dc.identifier.urihttp://hdl.handle.net/10019.1/11911
dc.subjectdiiodotyrosine
dc.subjectreceptor
dc.subjectanimal cell
dc.subjectdrug receptor binding
dc.subjectdrug structure
dc.subjectliver
dc.subjectliver cell nucleus
dc.subjectnonhuman
dc.subjectph
dc.subjectpriority journal
dc.subjectrat
dc.subjectDiiodothyronines
dc.subjectHydrogen-Ion Concentration
dc.subjectIons
dc.subjectModels, Theoretical
dc.subjectNuclear Proteins
dc.subjectReceptors, Thyroid Hormone
dc.subjectStructure-Activity Relationship
dc.subjectThyronines
dc.subjectTriiodothyronine
dc.titleExperimental values of the ionization constants for L-3,5-di-iodotyrosine and a model for ionic interactions of thyroid hormone (T3) and its nuclear receptor
dc.typeArticle
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