Bacteriocin T8, a novel class IIa sec-dependent bacteriocin produced by Enterococcus faecium T8, isolated from vaginal secretions of children infected with human immunodeficiency virus

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dc.contributor.authorDe Kwaadsteniet M.
dc.contributor.authorFraser T.
dc.contributor.authorVan Reenen C.A.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:56:32Z
dc.date.available2011-05-15T15:56:32Z
dc.date.issued2006
dc.description.abstractEnterococcus faecium T8, isolated from vaginal secretions of children with human immunodeficiency virus, produces a class IIa sec-dependent bacteriocin that is structurally different from three other class IIa sec-dependent bacteriocins, i.e., enterocin P and an enterocin P-like bacteriocin, produced by Enterococcus faecium, and bacteriocin 31, produced by Enterococcus faecalis, and from a class III bacteriocin produced by E. faecalis. The genes encoding the bacteriocin, immunity protein, mobilization protein, and relaxase nuclease are located on a 7-kb plasmid. Bacteriocin T8 has a molecular mass of 5.1 kDa based on its DNA sequence, similar to the 5.0 kDa recorded for bacteriocin 31 but larger than the 4.6 kDa reported for enterocin P. At the amino acid level, bacteriocin T8 is 69% homologous to bacteriocin 31 and 47% homologous to enterocin P. Bacteriocin T8 is active against E. faecalis isolated from patients diagnosed with vaginosis, against Lactobacillus sakei, and against a Propionibacterium sp. The peptide is heat stable (60 min at 100°C) and remains active in phosphate buffer from pH 4.0 to 10.0. The mode of activity is bactericidal, as determined with E. faecalis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation72
dc.identifier.citation7
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.00436-06
dc.identifier.urihttp://hdl.handle.net/10019.1/9903
dc.subjectAmino acids
dc.subjectBacteria
dc.subjectDNA sequences
dc.subjectGenes
dc.subjectImmunology
dc.subjectPatient treatment
dc.subjectProteins
dc.subjectGenes encoding
dc.subjectImmunodeficiency virus
dc.subjectMolecular mass
dc.subjectPlasmids
dc.subjectViruses
dc.subjectbacteriocin
dc.subjectbacteriocin 31
dc.subjectbacteriocin t8
dc.subjectbuffer
dc.subjectenterocin P
dc.subjectenterocin p like bacteriocin
dc.subjectgene product
dc.subjectimmunity protein
dc.subjectmobilization protein
dc.subjectnuclease
dc.subjectphosphate
dc.subjectrelaxase nuclease
dc.subjectunclassified drug
dc.subjectbacterium
dc.subjectchild
dc.subjecthuman immunodeficiency virus
dc.subjecttoxin
dc.subjectacidity
dc.subjectalkalinity
dc.subjectamino acid sequence
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectbactericidal activity
dc.subjectchild
dc.subjectDNA sequence
dc.subjectEnterococcus faecium
dc.subjecthuman
dc.subjectHuman immunodeficiency virus
dc.subjectHuman immunodeficiency virus infected patient
dc.subjectHuman immunodeficiency virus infection
dc.subjectLactobacillus sakei
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectPropionibacterium
dc.subjectsequence analysis
dc.subjectsequence homology
dc.subjectthermostability
dc.subjectvagina secretion
dc.subjectvaginitis
dc.subjectAmino Acid Sequence
dc.subjectBacteriocins
dc.subjectBase Sequence
dc.subjectBody Fluids
dc.subjectChild
dc.subjectEnterococcus faecalis
dc.subjectEnterococcus faecium
dc.subjectFemale
dc.subjectHIV Infections
dc.subjectHIV-1
dc.subjectHumans
dc.subjectLactobacillus
dc.subjectMicrobial Sensitivity Tests
dc.subjectMolecular Sequence Data
dc.subjectPropionibacterium
dc.subjectVagina
dc.subjectEnterococcus faecalis
dc.subjectEnterococcus faecium
dc.subjectHuman immunodeficiency virus
dc.subjectLactobacillus sakei
dc.subjectPropionibacterium
dc.titleBacteriocin T8, a novel class IIa sec-dependent bacteriocin produced by Enterococcus faecium T8, isolated from vaginal secretions of children infected with human immunodeficiency virus
dc.typeArticle
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