Xylose utilisation by recombinant strains of Saccharomyces cerevisiae on different carbon sources

Simple item page
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorEliasson A.
dc.contributor.authorHobley T.
dc.contributor.authorHahn-Hagerdal B.
dc.date.accessioned2011-05-15T15:56:37Z
dc.date.available2011-05-15T15:56:37Z
dc.date.issued1999
dc.description.abstractAutoselective xylose-utilising strains of Saccharomyces cerevisiae expressing the xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes of Pichia stipitis were constructed by replacing the chromosomal FUR1 gene with a disrupted fur1::LEU2 allele. Anaerobic fermentations with 80 g l-1 D-xylose as substrate showed a twofold higher consumption of xylose in complex medium compared to defined medium. The xylose consumption rate increased a further threefold when 20 g l-1 D-glucose or raffinose was used as co-substrate together with 50 g l-1 D-xylose. Xylose consumption was higher with raffinose as co-substrate than with glucose (85% versus 71%, respectively) after 82 h fermentations. A high initial ethanol concentration and moderate levels of glycerol and acetic acid accompanied glucose as co- substrate, whereas the ethanol concentration gradually increased with raffinose as co-substrate with no glycerol and much less acetic acid formation.
dc.description.versionArticle
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation52
dc.identifier.citation6
dc.identifier.issn1757598
dc.identifier.urihttp://hdl.handle.net/10019.1/9952
dc.subjectacetic acid
dc.subjectalcohol
dc.subjectcarbon
dc.subjectglucose
dc.subjectglycerol
dc.subjectoxidoreductase
dc.subjectraffinose
dc.subjectxylose
dc.subjectanaerobic metabolism
dc.subjectarticle
dc.subjectgene disruption
dc.subjectgene expression
dc.subjectnonhuman
dc.subjectpichia stipitis
dc.subjectsaccharomyces cerevisiae
dc.subjectAcetic acid
dc.subjectAnaerobiosis
dc.subjectChromatography, High Pressure Liquid
dc.subjectD-Xylulose Reductase
dc.subjectEndo-1,4-beta Xylanases
dc.subjectEthanol
dc.subjectFungal proteins
dc.subjectGlucose
dc.subjectGlycerol
dc.subjectPlasmids
dc.subjectRaffinose
dc.subjectRecombination, Genetic
dc.subjectSaccharomyces cerevisiae
dc.subjectSugar alcohol dehydrogenases
dc.subjectTime factors
dc.subjectXylose
dc.subjectXylosidases
dc.subjectPichia stipitis
dc.subjectSaccharomyces cerevisiae
dc.subjectTrixis
dc.titleXylose utilisation by recombinant strains of Saccharomyces cerevisiae on different carbon sources
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications