Survey of 3-alkyl-2-methoxypyrazine content of south african sauvignon blanc wines using a novel LC-APCI-MS/MS method

dc.contributor.authorAlberts P.
dc.contributor.authorStander M.A.
dc.contributor.authorPaul S.O.
dc.contributor.authorDe Villiers A.
dc.date.accessioned2011-05-15T15:56:50Z
dc.date.available2011-05-15T15:56:50Z
dc.date.issued2009
dc.description.abstractAn LC-MS/MS method for the trace-level determination of 3-alkyl-2-methoxypyrazines in Sauvignon blanc wines is described. 3-lsobutyl-2-methoxypyrazine (IBMP), 3-isopropyl-2-methoxypyrazine (IPMP) and 3-seobutyl-2-methoxypyrazine (SBMP) were analyzed by reversed phase liquid chromatography coupled to atmospheric pressure chemical ionization, as electrospray ionization was found to suffer from matrix quenching effects. A sample preparation method involving distillation of wine followed by solvent extraction and sufficient preconcentration was developed. Limits of detection and quantification for all three analytes were 0.03 ng/L and 0.10 ng/L, respectively, making the method more sensitive than gas chromatographic methods. IBMP was found to be the most abundant congener in South African Sauvignon blanc wines, with concentrations varying between 0.40 and 44 ng/L in 575 samples. IPMP and SBMP levels varied from <0.03 to 3.9 and <0.03 to 3.2 ng/L, respectively. Statistical investigation indicated no clear correlation between methoxypyrazine content and either geographical origin or vintage. The method was also successfully applied for the quantitation of IBMP in five additional South African wine varieties, including three red wine cultivars. The developed method represents a powerful new tool for the in-depth investigation of these important wine aroma constituents. © 2009 American Chemical Society.
dc.description.versionArticle
dc.identifier.citationJournal of Agricultural and Food Chemistry
dc.identifier.citation57
dc.identifier.citation20
dc.identifier.issn218561
dc.identifier.other10.1021/jf9026475
dc.identifier.urihttp://hdl.handle.net/10019.1/10066
dc.subject2 isobutyl 3 methoxypyrazine
dc.subject2-isobutyl-3-methoxypyrazine
dc.subjectpyrazine derivative
dc.subjectarticle
dc.subjectevaluation
dc.subjectliquid chromatography
dc.subjectmethodology
dc.subjectSouth Africa
dc.subjecttandem mass spectrometry
dc.subjectwine
dc.subjectChromatography, Liquid
dc.subjectPyrazines
dc.subjectSouth Africa
dc.subjectTandem Mass Spectrometry
dc.subjectWine
dc.titleSurvey of 3-alkyl-2-methoxypyrazine content of south african sauvignon blanc wines using a novel LC-APCI-MS/MS method
dc.typeArticle
Files