Intracytoplasmic sperm injection with testicular spermatozoa in men with azoospermia

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dc.contributor.authorWindt M.-L.
dc.contributor.authorCoetzee K.
dc.contributor.authorKruger T.F.
dc.contributor.authorMenkveld R.
dc.contributor.authorVan der Merwe J.P.
dc.date.accessioned2011-05-15T16:16:50Z
dc.date.available2011-05-15T16:16:50Z
dc.date.issued2002
dc.description.abstractPurpose: The aim of the study was to gain an insight into the optimal management of the infertile couple with the husband suffering from azoospermia. Methods: One hundred and forty-two intracytoplasmic sperm injection (ICSI) cycles performed with testicular extracted spermatozoa were retrospectively analysed. The following factors were investigated for their possible influence on fertilization, cleavage, damage, pregnancy, and ongoing pregnancy rates; the use of fresh, cryopreserved, and preincubated (24 h) spermatozoa and the etiology of the husbands' azoospermia (obstructive and nonobstructive). All microinjections were performed with apparently normal spermatozoa-a head with a tail of normal length. In 116 cycles at least two embryos were available for transfer. Results: The overall fertilization, clinical pregnancy, and ongoing pregnancy rates obtained for the 116 cycles were 65.0, 30.2, and 22.4% respectively. Similar outcomes were obtained for cycles using fresh testicular and cryopreserved testicular spermatozoa. Similarly, no significant differences were obtained between the cycles using spermatozoa from obstructive or nonobstructive azoospermic patients. An increase in motility after a 24-h preincubation was observed, and although this group was relatively small (n = 17), a significant improvement in fertilization (73.7%) and pregnancy (53.9%) rate was obtained when the testicular sample was preincubated for 24 h. This improvement prevailed in the obstructive azoospermic group, but was less pronounced in nonobstructive patients. Conclusions: This study shows that the outcome of fresh and frozen-thawed testicular spermatozoa in ICSI is comparable, obstructive and nonobstructive etiologies perform the same, and that preincubation of testicular spermatozoa results in increased fertilization and pregnancy rates. All testicular biopsies are therefore performed the day before oocyte retrieval, superfluous spermatozoa cryopreserved, and the remaining testicular homogenate preincubated for the 24 h prior to oocyte retrieval. With this regime, most azoospermic patients are treated successfully, irrespective of the use of fresh or frozen-thawed spermatozoa from obstructive or nonobstructive cases.
dc.description.versionArticle
dc.identifier.citationJournal of Assisted Reproduction and Genetics
dc.identifier.citation19
dc.identifier.citation2
dc.identifier.issn10580468
dc.identifier.other10.1023/A:1014487412975
dc.identifier.urihttp://hdl.handle.net/10019.1/13958
dc.subjectarticle
dc.subjectazoospermia
dc.subjectcell damage
dc.subjectcryopreservation
dc.subjectfertilization
dc.subjectfrozen section
dc.subjecthuman
dc.subjecthuman cell
dc.subjectincubation time
dc.subjectintracytoplasmic sperm injection
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmale infertility
dc.subjectoocyte cleavage
dc.subjectoocyte transport
dc.subjectoutcomes research
dc.subjectpregnancy rate
dc.subjectpriority journal
dc.subjectrisk factor
dc.subjectsemen analysis
dc.subjectsperm preservation
dc.subjectspermatozoon
dc.subjecttestis
dc.subjecttestis biopsy
dc.subjectthawing
dc.subjectCryopreservation
dc.subjectEmbryo Transfer
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectOligospermia
dc.subjectPregnancy
dc.subjectPregnancy Rate
dc.subjectRetrospective Studies
dc.subjectSemen Preservation
dc.subjectSperm Injections, Intracytoplasmic
dc.subjectSpermatozoa
dc.subjectTestis
dc.subjectTreatment Outcome
dc.titleIntracytoplasmic sperm injection with testicular spermatozoa in men with azoospermia
dc.typeArticle
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