Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD
dc.contributor.advisor | Swart, Pieter | en_ZA |
dc.contributor.advisor | Snoep, Jacob Leendert | en_ZA |
dc.contributor.author | Van Schalkwyk, Frederick Josephus (Erick) | en_ZA |
dc.contributor.other | Stellenbosch University. Faculty of Science. Department of Biochemistry | en_ZA |
dc.date.accessioned | 2016-03-09T14:54:15Z | |
dc.date.available | 2016-03-09T14:54:15Z | |
dc.date.issued | 2016-03 | |
dc.description.abstract | ENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using pregnenolone and progesterone as substrates ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine CYP17 ; how the time-dependent metabolism of pregnenolone, 17α-hydroxypregnenolone and dehydroepiandrosterone by ovine 3β-hydroxysteroid dehydrogenase (3β-HSD), was obtained ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine 3β-HSD | en_ZA |
dc.description.abstract | AFRIKAANSE OPSOMMING : Hierdie studie beskryf: hoe tyd-afhanklike eksperimentele data van die reaksies wat gekataliseer word deur skaap sitochroom P450 17α-hidroksielase/17,20-liase (CYP17) met pregnenoloon en progesteroon as substraat, verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap CYP17 gekataliseer word ; hoe die tyd-afhanklike metabolisme van pregnenoloon, progesteroon en dehidroepiandrosteroon wat gekataliseer word deur skaap 3β-hidroksiesteroïd dehidroginase (3β-HSD), verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap 3β-HSD gekataliseer word | af_ZA |
dc.description.uri | Thesis (MSc)--Stellenbosch University, 2016 | en_ZA |
dc.format.extent | xiii, 125 pages : illustrations (chiefly colour) | en_ZA |
dc.identifier.uri | http://hdl.handle.net/10019.1/98727 | |
dc.language.iso | en_ZA | en_ZA |
dc.publisher | Stellenbosch : Stellenbosch University | en_ZA |
dc.rights.holder | Stellenbosch University | en_ZA |
dc.subject | Steroid hormones | en_ZA |
dc.subject | Biosynthesis -- Models | en_ZA |
dc.subject | Steroidogenesis -- Models | en_ZA |
dc.subject | Adrenocortical hormones -- Models | en_ZA |
dc.subject | Kidneys -- Physiology | en_ZA |
dc.subject | Sheep -- Physiology | en_ZA |
dc.subject | UCTD | en_ZA |
dc.title | Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD | en_ZA |
dc.type | Thesis | en_ZA |