Effect of ethanol on [3H]dopamine release on rat nucleus accumbens and striatal slices

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dc.contributor.authorRuswel V.A.
dc.contributor.authorLamm M.C.L.
dc.contributor.authorTaljaard J.J.F.
dc.date.accessioned2011-05-15T16:17:28Z
dc.date.available2011-05-15T16:17:28Z
dc.date.issued1988
dc.description.abstractEthanol (10-200 mM) transiently increased tritium overflow from superfused rat nucleus accumbens slices previously incubated with [3H]dopamine (DA) and [14C]choline. The effect was greater in striatal tissue and did not appear to be a non-specific membrane effect since [14C]acetylcholine (ACh) release was not affected. Lack of antagonism by picrotoxin suggested that γ-aminobutyric (GABA) receptors were not involved. Calcium was not a requirement and the DA uptake blocker, nomifensine, was without effect. Ethanol appeared to be causing [3H]DA release into the cytoplasm. K+-stimulated release of [3H]DA and [14C]ACh from nucleus accumbens and striatal slices was not affected. Clonidine-mediated inhibition of the K+-evoked release of [3H]DA remained unaltered. Ethanol attenuated the isoproterenol-induced enhancement of [3H]DA release. Ethanol therefore appeared to interact with components of the DA terminal causing a transient increase in the release of neurotransmitter without impairing K+-evoked release but apparently interfering with the isoproterenol-induced effect.
dc.identifier.citationNeurochemical Research
dc.identifier.citation13
dc.identifier.citation5
dc.identifier.issn03643190
dc.identifier.urihttp://hdl.handle.net/10019.1/14233
dc.subjectalcohol
dc.subjectclonidine
dc.subjectisoprenaline
dc.subjectnomifensine
dc.subjectpicrotoxin
dc.subjectradioisotope
dc.subjectdiagnostic agent
dc.subjectdopamine
dc.subjecttritium
dc.subjectacetylcholine release
dc.subjectanimal cell
dc.subjectcorpus striatum
dc.subjectdepolarization
dc.subjectdopamine release
dc.subjectnonhuman
dc.subjectnucleus accumbens
dc.subjectrat
dc.subjecttissue slice
dc.subjectanimal
dc.subjectarticle
dc.subjectdose response
dc.subjectin vitro study
dc.subjectmale
dc.subjectmetabolism
dc.subjectrat strain
dc.subjectseptum nucleus
dc.subjectAnimal
dc.subjectClonidine
dc.subjectCorpus Striatum
dc.subjectDopamine
dc.subjectDose-Response Relationship, Drug
dc.subjectEthanol
dc.subjectIn Vitro
dc.subjectIsoproterenol
dc.subjectMale
dc.subjectNucleus Accumbens
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectSeptal Nuclei
dc.subjectSupport, Non-U.S. Gov't
dc.subjectTritium
dc.titleEffect of ethanol on [3H]dopamine release on rat nucleus accumbens and striatal slices
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