Effect of rapid dilution of semen on sperm-bound autoantibodies

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dc.contributor.authorWindt M.-L.
dc.contributor.authorMenkveld R.
dc.contributor.authorKruger T.F.
dc.contributor.authorVan der Merwe J.P.
dc.contributor.authorLombard C.J.
dc.date.accessioned2011-05-15T16:15:36Z
dc.date.available2011-05-15T16:15:36Z
dc.date.issued1989
dc.description.abstractThe effect of rapid dilution of autoantibody-positive semen on sperm-bound antibodies was studied in 12 male patients (0-100% IgA and 10-100% IgG). The direct immunobead test (IBT) and the sperm cervical mucus contact (SCMC) test were used to detect sperm-bound antibodies on spermatozoa before and after rapid dilution and swim-up in HAM F-10 + 10% human blood serum. All patients tested had normal semen parameters. Sperm-bound antibodies detected after swim-up of semen samples ejaculated into 15 ml HAM F-10 + 10% serum (diluted) did not differ significantly from undiluted samples. Most values were lower (IBT and SCMC) but were not statistically significant. Sperm motility, forward progression, and morphology were statistically improved. Pregnancies resulting from washed semen (diluted) in combination with artificial insemination (AIH) or gamete intrafallopian tube transfer (GIFT) took place in spite of antibodies still present on the sperm membrane and can probably be attributed to improved semen quality, minimizing of ovum-sperm distance, and cervical mucus elimination.
dc.description.versionArticle
dc.identifier.citationArchives of Andrology
dc.identifier.citation22
dc.identifier.citation3
dc.identifier.issn01485016
dc.identifier.urihttp://hdl.handle.net/10019.1/13408
dc.subjectantibody
dc.subjectadult
dc.subjectartificial insemination
dc.subjectclinical article
dc.subjecthuman
dc.subjectmale
dc.subjectmale infertility
dc.subjectpriority journal
dc.subjectsperm
dc.subjectAutoantibodies
dc.subjectCell Membrane
dc.subjectFertilization
dc.subjectHuman
dc.subjectImmunoassay
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin G
dc.subjectMale
dc.subjectSemen
dc.subjectSpermatozoa
dc.titleEffect of rapid dilution of semen on sperm-bound autoantibodies
dc.typeArticle
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