Adhesion of Lactobacillus plantarum 423 and Lactobacillus salivarius 241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum

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dc.contributor.authorMare L.
dc.contributor.authorWolfaardt G.M.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:59:24Z
dc.date.available2011-05-15T15:59:24Z
dc.date.issued2006
dc.description.abstractAims: To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. Methods and Results: Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combination, were administered to 1-, 14- and 28-day-old (postweaned) piglets. According to results obtained by fluorescent in situ hybridization (FISH), Lact. plantarum 423 adhered strongly to the ileum and posterior colon and Lact. salivarius 241 to the duodenum in preweaned piglets. High numbers of strain 241 were recorded in the duodenum and posterior colon of postweaned piglets, whereas strain 423 remained localized to the ileum. Lowering in Enterococcus faecalis cell numbers were recorded when preweaned piglets were challenged with strain 241. Plantaricin 423 was produced for 96 h in the ileum section of a gastro-intestinal model. Conclusions: Lactobacillus plantarum 423 and Lact. salivarius 241 adhere to different sections of the intestinal tract, depending on the piglet's age. Ent. faecalis were inhibited in vivo, probably by plantaricin 423. Significance and Impact of the Study: Fluorescent in situ hybridization proved valuable in the detection of probiotic bacteria adhered to the intestine. This is the first report of bacteriocin production in a model simulating the porcine gastro-intestinal tract. © 2006 The Society for Applied Microbiology.
dc.description.versionArticle
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation100
dc.identifier.citation4
dc.identifier.issn13645072
dc.identifier.other10.1111/j.1365-2672.2006.02835.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11158
dc.subjectbacteriocin
dc.subjectprobiotic agent
dc.subjectbacterial disease
dc.subjectcolonization
dc.subjectdigestive system
dc.subjectpig
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectcell count
dc.subjectcolon
dc.subjectduodenum
dc.subjectEnterococcus faecalis
dc.subjectfluorescence in situ hybridization
dc.subjectileum
dc.subjectintestine
dc.subjectLactobacillus plantarum
dc.subjectLactobacillus salivarius
dc.subjectnonhuman
dc.subjectswine
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectBacterial Adhesion
dc.subjectBacteriocins
dc.subjectColon
dc.subjectColony Count, Microbial
dc.subjectDuodenum
dc.subjectEnterococcus faecalis
dc.subjectGastrointestinal Tract
dc.subjectIleum
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectJejunum
dc.subjectLactobacillus
dc.subjectLactobacillus plantarum
dc.subjectProbiotics
dc.subjectSpecies Specificity
dc.subjectSwine
dc.subjectBacteria (microorganisms)
dc.subjectEnterococcus faecalis
dc.subjectLactobacillus plantarum
dc.subjectLactobacillus salivarius
dc.subjectSuidae
dc.subjectSus scrofa
dc.titleAdhesion of Lactobacillus plantarum 423 and Lactobacillus salivarius 241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum
dc.typeArticle
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