Design, fabrication and testing of an electrochemically based DNA biosensor for detection of Coniella Granati fungal disease of pomegranate fruit and aster yellows bacterial grapevine disease

Simple item page
dc.contributor.advisorPerold, W. J.en_ZA
dc.contributor.advisorOpara, U. L.en_ZA
dc.contributor.advisorBurger, J.en_ZA
dc.contributor.authorMadufor, Kennedy John Ndubuisien_ZA
dc.contributor.otherStellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering.en_ZA
dc.date.accessioned2019-02-21T12:20:40Z
dc.date.accessioned2019-04-17T08:17:13Z
dc.date.available2019-02-21T12:20:40Z
dc.date.available2019-04-17T08:17:13Z
dc.date.issued2019-04
dc.descriptionThesis (MEng)--Stellenbosch University, 2019.en_ZA
dc.description.abstractENGLISH ABSTRACT: A portable low cost electrochemical DNA potentiostat biosensor was investigated for the detection of Coniella granati pathogenic disease of pomegranate fruit, using linear biotinylated DNA probes, immobilized on streptavidin modified screen printed carbon electrode. A cyclic voltammetric based electrochemical technique was chosen to detect the presence of Coniella granati pathogenic disease. A modified streptavidin screen printed carbon electrode was selected as a transducer, with the biotinylated probe DNA immobilized non-covalently on the electrode workspace. The streptavidin-biotinylated probe DNA was validated by the use of streptavidin coated magnetic beads, with the biotinylated probe DNA in a bind or wash buffer solution. A portable electrochemical potentiostat was designed and fabricated to electronically detect the change in current peak due to the electrochemical reduction and oxidation reaction of the DNA samples, after the immobilization occurred. A potassium ferricyanide solution was used to perform a functionality sensor test with the portable potentiostat. The expected change in current peaks due to the redox reaction of the sample was observed. The operation of the sensor and portable potentiostat was then verified by successfully measuring different DNA concentrations of aster yellows and Coniella granati. The results confirmed that the potentiostat biosensor is capable to detect plant disease successfully.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: 'n Draagbare, laekoste elektrochemiese DNS potensiostaat biosensor is ondersoek vir die opspoor van die siekte Coniella granati in pomogranate, deur gebruik te maak van lineêre DNS probes wat deur middel van biotin-streptavadin koppelings geïmmobiliseer word op gedrukte koolstof elektrodes. 'n Sikliese voltammetries-gebaseerde tegniek is gebruik vir die opspoor van die siekte Coniella granati. 'n Gedrukte koolstofelektrode, gemodifiseer met streptavadin, is as omsetter gekies, met DNS probes wat deur middel van biotin nie-kovalent gekoppel word aan die elektrode. Die streptavadin-biotin koppeling van die DNS probe is geverifieer deur gebruik te maak van magnetiese krale, wat bedek is met streptavadin, in 'n bufferoplossing. Die draagbare elektrochemiese potensiostaat is ontwerp en vervaardig om die verandering van piekstroom as gevolg van die reduksie-oksidasie reaksie waar te neem nadat DNS hibridisasie plaasgevind het. 'n Kalium ferri-sianied oplossing is vervolgens gebruik om die funksionaliteit van die sensor met die draagbare potensiostaat te toets. Die verwagte vernadering in stroompieke as gevolg van die redoks reaksies van die steekproewe is waargeneem. Die werking van die sensor en die draagbare potensiostaat is geverifieer deur verskillende DNS konsentrasies van aster yellows en Coniella granati suksesvol te meet. Die resultate bevestig dat die potensiostaat biosensor in staat is om deteksie van plantsiektes suksesvol te doen.af_ZA
dc.format.extent111 pages : illustrationsen_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/105885
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch Universityen_ZA
dc.subjectElectrochemicals industryen_ZA
dc.subjectUCTDen_ZA
dc.subjectDNA -- Biosensorsen_ZA
dc.subjectConiella Granatien_ZA
dc.subjectFungal diseasesen_ZA
dc.subjectPomegranateen_ZA
dc.subjectAster yellowsen_ZA
dc.subjectBacterial diseases of plantsen_ZA
dc.subjectGrape leafroll virusen_ZA
dc.titleDesign, fabrication and testing of an electrochemically based DNA biosensor for detection of Coniella Granati fungal disease of pomegranate fruit and aster yellows bacterial grapevine diseaseen_ZA
dc.typeThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
madufor_design_2019.pdf
Size:
2.13 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Plain Text
Description:
Download
Collections
Masters Degrees (Electrical and Electronic Engineering)