Erythema induratum of Bazin: A clinicopathological study of 20 cases and detection of Mycobacterium tuberculosis DNA in skin lesions by polymerase chain reaction

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dc.contributor.authorSchneider J.W.
dc.contributor.authorJordaan H.F.
dc.contributor.authorGeiger D.H.
dc.contributor.authorVictor T.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorRossouw D.J.
dc.date.accessioned2011-05-15T16:15:19Z
dc.date.available2011-05-15T16:15:19Z
dc.date.issued1995
dc.description.abstractErythema induratum of Bazin (EIB) is a chronic, recurring panniculitis that is found predominantly on the legs of women with tuberculin hypersensitivity. A causal relationship between EIB and Mycobacterium tuberculosis remains elusive because of the absence of demonstrable organisms in skin lesions. We reviewed the clinicopathological features of 20 patients (all women) with positive Mantoux tests (1:10,000 dilution) and characteristic skin lesions of EIB that cleared up with combined antituberculous treatment. Histological examination of skin lesions confirmed panniculitis with varying combinations of granulomatous inflammation, primary vasculitis, and necrosis. Sections from 20 formalin-fixed, paraffin-embedded skin biopsies obtained from the 20 patients were submitted for polymerase chain reaction (PCR) using oligonucleotide primers for the detection of a 123-bp DNA fragment specific for the M. tuberculosis complex. M. tuberculosis DNA was identified in five of the 20 biopsies. Meticulous care was taken to prevent contamination as a source of false-positive results. Mycobacterial DNA was absent in all negative controls and in normal skin biopsies from purified protein derivative-positive patients with and without EIB. These results provide direct evidence that mycobacterial components are present in EIB lesions and strongly suggest that M. tuberculosis is involved in the pathogenesis of EIB.
dc.description.versionArticle
dc.identifier.citationAmerican Journal of Dermatopathology
dc.identifier.citation17
dc.identifier.citation4
dc.identifier.issn01931091
dc.identifier.urihttp://hdl.handle.net/10019.1/13283
dc.subjectbacterial dna
dc.subjectdna fragment
dc.subjectadolescent
dc.subjectadult
dc.subjectarticle
dc.subjectbacterium detection
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectdna content
dc.subjectfemale
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectmycobacterium tuberculosis
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectskin biopsy
dc.subjectskin tuberculosis
dc.subjectvasculitis
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAntitubercular Agents
dc.subjectDNA Primers
dc.subjectDNA, Bacterial
dc.subjectErythema Induratum
dc.subjectFemale
dc.subjectFixatives
dc.subjectFormaldehyde
dc.subjectGranuloma
dc.subjectHuman
dc.subjectLeg Dermatoses
dc.subjectMicrotomy
dc.subjectMiddle Age
dc.subjectMycobacterium tuberculosis
dc.subjectNecrosis
dc.subjectPanniculitis
dc.subjectParaffin Embedding
dc.subjectPolymerase Chain Reaction
dc.subjectSkin
dc.subjectSupport, Non-U.S. Gov't
dc.subjectTuberculin Test
dc.subjectVasculitis
dc.titleErythema induratum of Bazin: A clinicopathological study of 20 cases and detection of Mycobacterium tuberculosis DNA in skin lesions by polymerase chain reaction
dc.typeArticle
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