A sensitive urinary lipoarabinomannan test for tuberculosis

Hamasur, Beston ; Bruchfeld, Judith ; Van Helden, Paul ; Kallenius, Gunilla ; Svenson, Stefan (2015)

CITATION: Hamasur, B., et al. 2015. A sensitive urinary lipoarabinomannan test for tuberculosis. PLoS ONE, 10(4):1-11, doi:10.1371/journal.pone.0123457.

The original publication is available at http://journals.plos.org/plosone

Article

We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIVnegative TB patients was very low.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/99436
This item appears in the following collections: