Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus
Van Zyl L.J.
Three large cryptic plasmids from different isolates of Acidithiobacillus caldus were rescued by using an in vitro transposition system that delivers a kanamycin-selectable marker and an Escherichia coli plasmid origin of replication. The largest of the plasmids, the 65-kb plasmid pTcM1, was isolated from a South African A. caldus strain, MNG. This plasmid was sequenced and compared to that of pTcF1 (39 kb, from strain "f," South Africa) and pC-SH12 (29 kb, from strain C-SH12, Australia). With the exception of a 2.7-kb insertion sequence, pC-SH12 appears to represent the DNA common to all three plasmids and includes a number of accessory genes plus the plasmid "backbone" containing the replication region. The two larger plasmids carry, in addition, a number of insertion sequences of the ISL3 family and a composite transposon related to the Tn21 subfamily containing a highly mosaic region within the borders of the inverted repeats. Genes coding for arsenic resistance, plasmid mobilization, plasmid stability, and a putative restriction-modification system occur within these mosaic regions. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Arsenic, Escherichia coli, Gene encoding, Genes, Nonmetals, Nucleic acids, Organic acids, Programming theory, Strain, Sulfur, System stability, Acidithiobacillus caldus, American society, Arsenic resistance, Australia, Cryptic plasmids, Genes coding, In-vitro, Insertion sequence (IS), Kanamycin (KM), Plasmid stability, Restriction modification, South Africa (SA), Sulfur oxidizing bacterium (SOB), Health, DNA fragment, arsenic, bacterium, biomarker, comparative study, DNA, gene expression, oxidation, plasmid, sulfur, Acidithiobacillus caldus, article, bacterial strain, controlled study, gene sequence, gene structure, nonhuman, nucleotide sequence, plasmid, replicon, species difference, sulfur oxidizing bacterium, transposon, Acidithiobacillus, Australia, Chromosome Mapping, Cloning, Molecular, DNA Transposable Elements, DNA, Bacterial, Open Reading Frames, Plasmids, Replicon, Restriction Mapping, Sequence Analysis, DNA, South Africa, Sulfur-Reducing Bacteria, Acidithiobacillus caldus, Bacteria (microorganisms), Escherichia coli
Applied and Environmental Microbiology