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Now showing items 1-14 of 14
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Analysis of sugar accumulation under cold stress in Physcomitrium patens
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Plants adapt to cold temperatures through the process of cold acclimation. Key experimental observations indicate that one of the common elements in cold acclimation are the accumulation of various ... -
The Arabidopsis GolS1 promotor as a potential biosensor for heat stress and fungal infection?
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Galactinol (Gol) has classically been considered to serve as a galactose donor during the biosynthesis of raffinose family oligosaccharides (RFOs). These sucrosyl oligosaccharides have been well characterised ... -
Characterisation of the glycoside hydrolase domain of a novel bi-functional metagenomic clone for use in the biofuel production industry
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH ABSTRACT: The current practice of 1st generation biofuel production is marred by several hurdles, namely concerns over food security and the moral dilemma created by using edible feedstocks as a biofuel ... -
Development of a hairy root bioreactor from Stevia rebaudiana to produce steviol glycosides
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH SUMMARY: Extracts from the leaves of Stevia rebaudiana, a plant native to South America, have been used as natural sweetener for centuries. With the global epidemic of obesity linked to increased prevalence of ... -
Functional identification of a putative stachyose synthase (StaS, Medtr7g106910.1) from Medicago truncatula, by overexpression in the Arabidopsis stachyose deficient double mutant atrs4/atrs5
(Stellenbosch : Stellenbosch University, 2018-11)ENGLISH ABSTRACT: The Raffinose Family Oligosaccharides (RFOs; Suc-Galn, 13 < n ≥ 1) are α1,6- galactosyl extensions of sucrose occurring exclusively in the plant kingdom and some photoautotrophic bacteria. This unique ... -
Functional identification of the chloroplastic raffinose transporter from arabidopsis
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: In plants, the raffinose family oligosaccharides (RFOs) have a wide range of functions, ranging from membrane trafficking to participation in signal transduction processes. Their most well characterized ... -
Functional roles of raffinose family oligosaccharides: Arabidopsis case studies in seed physiology, biotic stress and novel carbohydrate engineering
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: The raffinose family of oligosaccharides (RFOs) are α1,6-galactosyl extensions of sucrose (Suc-Galn) unique to the plant kingdom. Their biosynthesis is mediated via α1,6-galactosyltransferases which ... -
Identification and biochemical characterisation of an aryl β-glucosidase isolated from a cellulose-acetate rich environment via a functional metagenomic approach
(Stellenbosch : Stellenbosch University, 2018-12)ENGLISH ABSTRACT: Functional metagenomics has established itself as an indispensable tool in the search for novel genes by accessing the genetic information of previously unculturable microbes. These novel genes are ... -
Identification of a cellulolytic active fungal strain, isolated from a cigarette waste microenvironment, showing preference for growth on cellulose acetate
(Stellenbosch : Stellenbosch University, 2020-12)ENGLISH ABSTRACT: Globally, environmental pollution such as greenhouse gases and microplastics are ubiquitous throughout nature. Long-term negative environmental effects and bioaccumulation of anthropogenic compounds ... -
Mutagenesis studies of a glycoside hydrolase family 2 enzyme
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: Galactooligosaccharides are produced by the transglycosylation activity of β-galactosidases (β-gal, EC 3.2.1.23) when utilising lactose as a substrate. They have emerged as important constituents used in ... -
Site-directed mutagenesis studies on a novel dual domain β-galactosidase/ β-glucosidase open reading frame identified from a dairy run-off metagenome
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Glycosyl hydrolases (GHs, EC 3.2.1.-) are currently represented by 171 distinct families (www.CAZY.org) and catalyse the hydrolysis of glycosidic bonds present in various biological molecules termed ... -
Targeted metabolite profiling of pigeon pea (Cajanus cajan) seeds and, toward product (falafel) development
(Stellenbosch : Stellenbosch University, 2022-04)ENGLISH ABSTRACT: Global food systems are currently under pressure, not only to meet the demand for food production, but also toward diversifying our selection of crops based on their nutrient profile. Furthermore, the ... -
The use of phylogenetic reconstruction as a predictive tool to functionally identify raffinose family oligosaccharide (RFO) producing glycosyltransferases
(Stellenbosch : Stellenbosch University, 2022-04)ENGLISH ABSTRACT: Carbohydrate active enzymes (CAZymes) are numerous and diverse enzymes that are involved with the transport, synthesis, and catalysis of carbohydrates. All known and predicted CAZymes are housed on the ... -
Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: Late Embryogenesis Abundant (LEA) genes have been irrefutably linked to the osmotic stress response since their initial discovery in maturing cotton seeds. They have since been reported from a multitude ...
