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Comparison of diagnostic criteria of tuberculous meningitis in human immunodeficiency virus-infected and uninfected children
(2006)INTRODUCTION: Tuberculous (TB) meningitis is sometimes difficult to diagnose in young children. The decision to start anti-TB treatment of TB meningitis is usually made on clinical grounds and results of special investigations, ... -
Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion
(1999)The incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ... -
Improvement in mycobacterial yield and reduced time to detection in pediatric samples by use of a nutrient broth growth supplement
(2009)There is an urgent need to improve the methods used for the bacteriological diagnosis of childhood mycobacterial disease. This study compared the mycobacterial yields and the times to detection (in days) of mycobacteria ...