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Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa

dc.contributor.authorJacobs, Graeme B.en_ZA
dc.contributor.authorBock, Stefanieen_ZA
dc.contributor.authorSchuch, Anitaen_ZA
dc.contributor.authorMoschall, Rebeccaen_ZA
dc.contributor.authorSchrom, Eva-Mariaen_ZA
dc.contributor.authorZahn, Julianeen_ZA
dc.contributor.authorReuter, Christianen_ZA
dc.contributor.authorPreiser, Wolfgangen_ZA
dc.contributor.authorRethwilm, Axelen_ZA
dc.contributor.authorEngelbrecht, Susanen_ZA
dc.contributor.authorKerkau, Thomasen_ZA
dc.contributor.authorBodem, Jochenen_ZA
dc.date.accessioned2013-07-03T08:18:52Z
dc.date.available2013-07-03T08:18:52Z
dc.date.issued2012-09-24
dc.identifier.citationJacobs, G. B. et al. 2012. Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa. Viruses, 4(9):1830-1843; doi:10.3390/v4091830.
dc.identifier.issn1999-4915 (online)
dc.identifier.otherdoi:10.3390/v4091830
dc.identifier.urihttp://hdl.handle.net/10019.1/81483
dc.descriptionCITATION: Jacobs, G. B. et al. 2012. Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa. Viruses, 4(9):1830-1843; doi:10.3390/v4091830.
dc.descriptionThe original publication is available at http://www.mdpi.com/journal/viruses
dc.description.abstractThe Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317.
dc.description.urihttp://www.mdpi.com/1999-4915/4/9/1830
dc.format.extent14 pages
dc.language.isoen_ZA
dc.publisherMDPI
dc.subjectHIV-1 subtype Cen_ZA
dc.subjectProviral plasmiden_ZA
dc.subjectViral replicationen_ZA
dc.subjectResistance assaysen_ZA
dc.subjectVirusses -- Variationsen_ZA
dc.subjectHIV (Viruses ) -- Researchen_ZA
dc.titleConstruction of a high titer infectious HIV-1 subtype C proviral clone from South Africaen_ZA
dc.typeArticle
dc.description.versionPublisher's version
dc.rights.holderAuthors retain copyright


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