'n Nuwe metode vir die bepaliog van serotonien in bloed en serebrospinale vloeistof
The original publication is available at http://www.samj.org.za
CITATION: Kriek, J. A., Bester, A. J. & Rossouw, J. J. 1979. 'n Nuwe metode vir die bepaliog van serotonien in bloed en serebrospinale vloeistof. South African Medical Journal, 55:260-262.
A new rapid method for the microdetermination of serotonin (5-hydroxytryptamine) in plasma and cerebrospinal fluid is described. A recovery coefficient of 98% was obtained in the present method, with the use of [3H]-hydroxytryptamine creatinine sulphate as an internal standard, compared with recovery coefficients of 31% and 66% for the two existing methods generally used for serotonin determination. This method involves a one-step sample deproteinization by perchloric acid, resulting in a high stability of serotonin.