Browsing Masters Degrees (Genetics) by Title
Now showing items 1-20 of 201
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The analysis and reduction of starch in sugarcane by silencing ADP-glucose pyrophosphorylase and over-expressing β-amylase
(Stellenbosch : University of Stellenbosch, 2007-12)Sugarcane is cultivated because of the high levels of sucrose it stores in its internodes. Starch metabolism has been a neglected aspect of sugarcane research despite the problems caused by it during sugarcane processing. ... -
Analysis of dextrin dextranase from Gluconobacter oxydans
(Stellenbosch : Stellenbosch University, 2008-12)Dextran is a high value glucose polymer used in medicine and an array of laboratory techniques. It is synthesised by lactic-acid bacteria from sucrose but has also reportedly been produced by Gluconobacter oxydans (G. ... -
Analysis of enzymes involved in starch phosphate metabolism
(Stellenbosch : University of Stellenbosch, 2009-12)ENGLISH ABSTRACT: This project examined the role of proteins in starch phosphate metabolism. The first part was aimed at the functional characterization of the SEX4, LSF1 and LSF2 genes in both plants and bacteria. ... -
Analysis of genes implicated in iron regulation in individuals presenting with primary iron overload in the South African population
(Stellenbosch : University of Stellenbosch, 2007-03)Hereditary haemochromatosis (HH), a common autosomal recessive disease, is characterized by increased iron absorption leading to progressive iron accumulation in organs such as the liver, heart and pancreas. In the South ... -
Analysis of genetic variants in the 5’ regulatory region of the ALAS1 gene in South African patients with Variegate Porphyria (VP)
(Stellenbosch : University of Stellenbosch, 2007-03)The porphyrias are a group of genetic disorders arising from mutations in either one of the final seven genes encoding the haeme synthesis enzymes. These disease-causing mutations lead to an enzyme deficiency that disrupts ... -
The analysis of glycogen phosphate and glucose-1,6-bisphosphate metabolism in escherichia coli
(Stellenbosch : Stellenbosch University, 2015-03)ENGLISH ABSTRACT: This thesis examined two aspects of E. coli carbon metabolism, the incorporation of covalently bound phosphate into glycogen as well as the manufacture of glucose-1,6-bisphosphate (GBP). In vitro analysis ... -
Analysis of intermediate carbon metabolism in strawberry plants
(Stellenbosch : Stellenbosch University, 2008-12)Strawberry (Fragaria x ananassa) fruit quality is largely determined by the relative amounts of sugars and organic acids present, as well as soluble solid content. This study had three components: 1) Characterisation of ... -
Analysis of schizophrenia susceptibility variants identified by GWAS : a bioinformatics and molecular genetics approach
(Stellenbosch : Stellenbosch University, 2014-12)ENGLISH ABSTRACT: Described as one of the costliest and most debilitating disorders, schizophrenia has proven to be among the greatest challenges for medical researchers. The disorder poses difficulties on all levels: from ... -
Analysis of the effects of the plant growth promoting substances GR24 and smoke water on abiotically stressed Nicotiana benthamiana seedlings
(Stellenbosch : Stellenbosch University, 2012-03)ENGLISH ABSTRACT: Almost all processes during the life of a plant are affected by the environment. Changes in phytohormone, metabolite and protein levels follow in response to changes in the environment. Plant growth ... -
Application of plant growth promoting substances and arbuscular mycorrhizal fungi for phytostabilisation of mine tailings
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT: This study focused on investigating methods of phytostabilisation of mine tailings operated by Palabora Copper in South Africa. Capping material and mine tailing at various sites of the mine were collected ... -
Approaches for the study of leaf carbohydrate metabolic compartmentation in arabidopsis thaliana
(Stellenbosch : University of Stellenbosch, 2010-12)ENGLISH ABSTRACT: The study of plants on a sub-cellular level is an important, yet challenging area and its application allows for novel insight into the understanding of metabolism and its regulation. In this study I ... -
The Arabidopsis GolS1 promotor as a potential biosensor for heat stress and fungal infection?
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Galactinol (Gol) has classically been considered to serve as a galactose donor during the biosynthesis of raffinose family oligosaccharides (RFOs). These sucrosyl oligosaccharides have been well characterised ... -
Assessing the cyto-genotoxic impacts of un-neutralised and pH-neutralised acid mine drainage on the human breast cancer cell line, MCF-7
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: The use of toxicity tests to evaluate the quality of streams affected by mixtures such as acid mine drainage (AMD), adds value to assessments whereby site-specific toxicological data may identify toxicants ... -
Assessment of yield traits between family groups of the cultured abalone (Haliotis midae) in South Africa
(Stellenbosch : Stellenbosch University, 2012-03)ENGLISH ABSTRACT: The abalone Haliotis midae is the most important aquaculture species in South Africa. The industry is dependent on export to Far Eastern markets in a variety of forms, including live, frozen, canned or ... -
Association of genetic variants and the susceptibility to abnormal involuntary movements and tardive dyskinesia (TD) in Xhosa schizophrenia patients
(Stellenbosch : University of Stellenbosch, 2007-03)No obvious explanations exist for the development of abnormal involuntary movements (AIM), but several hypotheses have been proposed for tardive dyskinesia (TD) development. Since TD seems to have a genetic basis, several ... -
Attempting to enhance sugarcane growth through genetic modification
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT: The current focus of plant breeding is to increase yield and meet the food and energy demands of a growing global population. Progress in this field of study is slow due to the complexity of yield, which ... -
Biophysical characterization of the mineral composition of seeds with varying genetic background including transgenic sorghum with reduced amounts of the storage protein kafirin
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH ABSTRACT: Cereals are globally recognised as a cornerstone of human nutrition, and as a result play a pivotal role in efforts to address food insecurity and malnutrition. In Africa, the highest rates of hunger ... -
Biopolymer gene discovery and characterization using metagenomic libraries
(Stellenbosch : Stellenbosch University, 2008-12)Traditional methods used for the discovery of novel genes have previously relied upon the ability to culture the relevant microbes and then demonstrate the activity of a specific enzyme. Although these methods have proved ... -
Biotechnological approaches for sugarcane enhancement – Drought tolerance and Sucrose accumulation
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: The application of biotechnological approaches can supplement traditional crop breeding strategies to combat the effects of adverse environmental conditions on plant production in order to bridge the gap ... -
Carbon turnover and sucrose metabolism in the culm of transgenic sugarcane producing 1-Kestose
(Stellenbosch : University of Stellenbosch, 2007-12)Carbon partitioning was investigated in sugarcane (Saccharum spp. hybrids) that was genetically modified with sucrose: sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) from Cynara scolymus. This enzyme catalyses the ...
