Now showing items 1-5 of 5
Engineering cellulolytic ability into bioprocessing organisms
Lignocellulosic biomass is an abundant renewable feedstock for sustainable production of commodities such as biofuels. The main technological barrier that prevents widespread utilization of this resource for production of ...
Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae alpha-amylase in industrial strains of Saccharomyces cerevisiae
(Kluwer (now part of Springer), 2002-01)
A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae alpha-amylase-encoding gene (LKA1). These transformants ...
Cloning of the Bacillus pumilus beta-xylosidase gene (xynB ) and its expression in Saccharomyces cerevisiae
A genomic DNA library of the bacterium Bacillus pumilus PLS was constructed and the beta-xylosidase gene (xynB) was amplified from a 3-kb genomic DNA fragment with the aid of the polymerase chain reaction technique. The ...
Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae
(American Society for Microbiology, 1995-04)
The XYN2 gene encoding the main Trichoderma reesei QM 6a endo-beta-1,4-xylanase was amplified by PCR from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was ...
A Chromogenic substrate for a β-xylosidase-coupled assay of α-glucuronidase
-Nitrophenyl 2-(4-O-methyl-α- -glucopyranuronosyl)-β- -xylopyranoside obtained on deesterification of 4-nitrophenyl 2-O-(methyl 4-O-methyl-α- -glucopyranosyluronate)-β- -xylopyranoside (Hirsch et al., Carbohydr. Res. 310, ...