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Sequence analysis of a Cowdria ruminantium lamdba (sic) GEM-11 clone
(Stellenbosch : Stellenbosch University, 2002-12)
ENGLISH ABSTRACT: Heartwater is a major threat to livestock in Africa due to its high mortality rate. The
intracellular nature of the causative organism, Cowdria ruminantium, makes it difficult to
study, hence an effective ...
UF membranes operated on paper machine wastewater : fouling tendencies and characterisation
(Stellenbosch : Stellenbosch University, 2001-12)
ENGLISH ABSTRACT: At the Mondi Kraft paper mill in Piet Retief, paper machine effluent is pre-treated by means
of dissolved air flotation (DAF) and a mierostrainer prior to ultrafiltration (UF). Despite the
rigorous ...
Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up
(Stellenbosch : Stellenbosch University, 2012-12)
ENGLISH ABSTRACT: This study describes:
a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin
b) Re-engineering / development of a new method for ...
The optimization of the extraction and purification of horseradish peroxidase from horseradish roots
(Stellenbosch : Stellenbosch University, 2012-12)
ENGLISH ABSTRACT: This study describes:
a) the optimization of the current industrial-scale extraction and purification of Horseradish
peroxidase from horseradish at BBI Enzymes, focussing on:
a. Raw material quality,
b. ...
Functional analysis and recombinant expression of a sea urchin G-string binding factor
(Stellenbosch : Stellenbosch University, 2001-12)
ENGLISH ABSTRACT: The sea urchin G-string binding factor 1 (suGF1) has previously been shown to bind with
high affinity and selectivity to stretches of contiguous deoxyguanosine residues, a DNA
motif found in the upstream ...
The effect of modulators of inflammation on hepatic acute phase proteins and metabolic enzymes
(Stellenbosch : University of Stellenbosch, 2010-03)
ENGLISH ABSTRACT:
Crosstalk exists between the stress- and immune-system and this crosstalk has pharmacological
importance in the use of glucocorticoids (GCs) as anti-inflammatory drugs for diseases such as
asthma and ...
Computational systems biology of sucrose accumulation in sugarcane
(Stellenbosch : University of Stellenbosch, 2006-03)
This thesis is about mathematical modelling of sucrose accumulation in the storage perenchyma of Saccharum officinarum (sugarcane) In 2001, Rohwer & Botha (76) published a kinetic model that described the defining feature ...
An investigation into the stress relieving properties of Sutherlandia frutescens : inhibition of steroidogenic cytochrome P450 enzymes
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study:
I. Investigates the influence of S. frutescens on the binding properties of cytochrome
P450-dependent enzymes in ovine adrenocortical mitochondria and microsomes,
demonstrating that S. ...
Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study describes:
I. The cloning of the CVP 19 gene and construction of the intracellular expression
vector pPIC3.5K-CYP19.
II. The transformation of the yeast, Pichia pastoris with the constructed ...
Effects of neutralising interleukin-6 on glucocorticoid-mediated adaptations to stress in rat skeletal muscle and liver
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study (2 x 2 factor design) describes an investigation into the physiological interaction
between the peripheral endocrine and cytokine systems after the organism has been
exposed to psychological ...