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An investigation into the stress relieving properties of Sutherlandia frutescens : inhibition of steroidogenic cytochrome P450 enzymes
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study:
I. Investigates the influence of S. frutescens on the binding properties of cytochrome
P450-dependent enzymes in ovine adrenocortical mitochondria and microsomes,
demonstrating that S. ...
Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study describes:
I. The cloning of the CVP 19 gene and construction of the intracellular expression
vector pPIC3.5K-CYP19.
II. The transformation of the yeast, Pichia pastoris with the constructed ...
A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1)
(Stellenbosch : University of Stellenbosch, 2005-12)
This study describes:
1. The cloning of baboon cytochrome P450 side-chain cleavage (CYP11A1) cDNA by in
vitro site-directed mutagenesis.
2. The identification and sequencing of three baboon CYP11A1 mutants: CYP11A1a,
CYP11A1b ...
An investigation into the bioactivity of Sutherlandia frutescens (Cancer bush)
(Stellenbosch : University of Stellenbosch, 2009-03)
Sutherlandia frutescens (S. frutescens), sub-species microphylla, is a member of the Fabacea
family and is used as a herbal remedy for the treatment of several ailments which include
influenza, diabetes, cancer, tuberculosis, ...
The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones
(Stellenbosch : Stellenbosch University, 2015-04)
ENGLISH ABSTRACT: This study describes:
1. Substrate binding assays investigating the effects of methanolic extracts of
unfermented and fermented Rooibos on the binding of natural substrates to ovine
adrenal microsomal ...
The influence of S. frutescens on adrenal cytochrome P450 11B-hydroxylase
(Stellenbosch : University of Stellenbosch, 2009-12)
ENGLISH ABSTRACT:
This study:
1. describes the preparation of a methanol extract of Sutherlandia frutescens and the HPLC
fractionation of the methanol extract.
2. investigates the influence of S. frutescens on the ...
An investigation into the catalytic activity of porcine cytochrome P450 17α-hydroxylase/17,20-lyase
(Stellenbosch : Stellenbosch University, 2014-04)
ENGLISH ABSTRACT: In this study, the effect of the amino acid residues at positions 40 and 407 on the catalytic activity of
porcine CYP17A1 was investigated. Porcine cofactor CYB5 was cloned from porcine liver tissue ...
The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes
(Stellenbosch : University of Stellenbosch, 2009-03)
This study:
1. Describes the preparation of unfermented and fermented rooibos methanol and aqueous
extracts.
2. Investigates the influence of unfermented and fermented rooibos methanol and aqueous
extracts on the binding ...
A biochemical and immunochemical study of ovine adrenal cytochrome P-450 reductase
(Stellenbosch : Stellenbosch University, 1992-03)
ENGLISH ABSTRACT: This study describes:
(a) the isolation · of ovine adrenal cytochrome P-450
reductase,
(b) the preparation of , antibodies against this enzyme and
(c) comparitive immunochemical studies with ovine' ...
The influence of biologically active compounds from Salsola tuberculatiformis Botsch. on steroid protein interactions
(Stellenbosch : Stellenbosch University, 1998)
ENGLISH ABSTRACT: This study forms part of an ongoing investigation into the syndrome of prolonged gestation in sheep and contraception in rats, caused by Namibian shrub, Salsola tuberculatiformis Botsch. Evidence was ...