Browsing Masters Degrees (Biochemistry) by Title
Now showing items 1-20 of 104
-
The age-old problem of pollution, its role in endocrine disruption and the current analytical technologies that can be employed to monitor and assess waste water treatment plants
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Water scarcity is a global problem and pollution of this valuable resource is a growing concern. South Africa is no exception. As part of an on-going study aimed at developing decentralized water treatment ... -
Applications of generalised supply-demand analysis
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Supply-demand analysis (SDA) is a tool that allows for the control, regulation and behaviour of metabolic pathways to be understood. In this framework, reactions are grouped into reaction blocks that ... -
An assessment of the mutation patterns in South African isolates of Potato leafroll virus and the expression of recombinant viral coat protein genes in Escherichia coli
(Stellenbosch : Stellenbosch University, 2007-03)ENGLISH ABSTRACT: Presently, the observed variation in symptoms of Potato leafroll virus (PLRV) infection in potato cultivars in South Africa cannot be reconciled with PLRV symptoms obtained 10-15 years ago, even if the ... -
Atomic force microscopy : a novel tool for the analysis of the mechanism of action of antimicrobial peptides on target membranes
(Stellenbosch : Stellenbosch University, 2003-03)ENGLISH ABSTRACT: Nanoscale visualisation of live cells and cellular components under physiological conditions has long been a goal in microscopy. The objective of this study was to validate the use of Atomic Force ... -
Biocatalytic preparation and characterization of alternative substrate of MshB, a mycothiol pathway enzyme
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: Mycobacterium tuberculosis (M. tuberculosis), the causative agent of tuberculosis, utilizes mycothiol (MSH) as the major low molecular weight thiol to protect itself against oxidative stress and thereby ... -
Biochemical and genetic characterization of bacteria isolated from diseased rainbow trout (Oncorhynchus mykiss) farmed in Lesotho and Mpumalanga province of South Africa
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH ABSTRACT: Rainbow trout farms in Mpumalanga Province, South Africa and Lesotho, have periodically suffered significant losses from infections caused by Gram-positive bacteria. Such outbreaks have hampered the ... -
A biochemical and immunochemical study of ovine adrenal cytochrome P-450 reductase
(Stellenbosch : Stellenbosch University, 1992-03)ENGLISH ABSTRACT: This study describes: (a) the isolation · of ovine adrenal cytochrome P-450 reductase, (b) the preparation of , antibodies against this enzyme and (c) comparitive immunochemical studies with ovine' ... -
A biochemical and immunological study of horseradish peroxidase
(Stellenbosch : University of Stellenbosch, 2009-12)ENGLISH ABSTRACT: This study describes: a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots, b) the characterization of various forms and components of the enzyme by cation-exchange ... -
A biochemical study of budbreak and plant growth regulators in table grapes
(Stellenbosch : Stellenbosch University, 2002-03)ENGLISH ABSTRACT: The cultivation of table grapes in the warmer areas of South Africa, indeed worldwide, is complicated by rest breaking problems in spring due to delayed budbreak. In order to overcome these problems ... -
The biosynthesis of adrenal C 11-oxy C21 steroids, implicated in 21-hydroxylase deficiency-21-desoxycortisol and 21 desoxycortisone and their downstream metabolism
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Adrenal C19 steroids are often implicated in numerous androgen dependent disease conditions. Androgen excess is the hallmark of 21-hydroxylase deficiency (21OHD) fuelled by an increase in the production ... -
The characterisation of the catalytic activity of human steroid 5α-reductase towards novel C19 substrates
(Stellenbosch : Stellenbosch University, 2015-02-25)ENGLISH ABSTRACT: This study describes: • The UPLC-MS/MS analyses and quantification of novel 5α-reduced steroids using response factors. • The kinetic characterisation of human steroid 5α-reductase type 1 (SRD5A1), expressed ... -
The characterization of 17β-hydroxysteroid dehydrogenase type 2 (17βHSD2) activity towards novel C19 substrates.
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Castration resistant prostate cancer (CRPC) is an androgen dependent disease driven by the intratumoural metabolism of adrenal androgen precursors to potent androgens. The alternative 5α-dione pathway ... -
Characterization of natural antimicrobial peptides adsorbed to different matrices
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: Biofouling is the attachment and biofilm formation that leads to negative repercussions such as persistent post-harvest infections, infections obtained from medical implants and continual surface ... -
Cloning and functional characterization of thiol disulfide interchange system proteins from Staphylococcus aureus
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: The 21st century has seen the re-emergence of infectious diseases thought previously conquered by antibiotics. The human pathogen, Staphylococcus aureus, is one such example, with global reports listing ... -
A comparative analysis of mathematical models for HIV epidemiology
(Stellenbosch : Stellenbosch University, 2015-04)ENGLISH ABSTRACT: HIV infection is one of the world’s biggest health problems, with millions of people infected worldwide. HIV infects cells in the immune system, where it primarily targets CD4+ T helper cells and without ... -
Comparative secretome analysis of normal prostate and prostate cancer cell models
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH SUMMARY: Prostate cancer is the second most common cancer among men. The prostate specific antigen (PSA) was the first biomarker identified for the diagnosis of this prevalent disease. Although this biomarker ... -
Comparison of progress curve analysis and initial rate kinetics for the estimation of identifiable enzyme kinetic parameters : a case study using two glycolytic enzymes from Sulfolobus solfataricus
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Obtaining accurate parameter value estimations is imperative to studying enzyme kinetics. It is also important that these parameter values be identifiable. The nonidentifiability of parameter estimations ... -
Computational systems biology of sucrose accumulation in sugarcane
(Stellenbosch : University of Stellenbosch, 2006-03)This thesis is about mathematical modelling of sucrose accumulation in the storage perenchyma of Saccharum officinarum (sugarcane) In 2001, Rohwer & Botha (76) published a kinetic model that described the defining feature ... -
Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using pregnenolone and ... -
Construction and validation of a detailed kinetic model of glycolysis in asexual Plasmodium falciparum : a feasibility study
(Stellenbosch : University of Stellenbosch, 2009-12)ENGLISH ABSTRACT: In Africa alone, Plasmodium, the causative agent of malaria is estimated to kill a child, under the age of five every thirty seconds140. The ability of the parasite to rapidly attain resistance, has ...
