Browsing Doctoral Degrees (Microbiology) by Title
Now showing items 1-20 of 54
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Assessing the occurrence and mechanisms of horizontal gene transfer during wine making
(2009-12)ENGLISH ABSTRACT: Saccharomyces cerevisiae is the most commonly used organism in many fermentation-based industries including baking and the production of single cell proteins, biofuel and alcoholic beverages. In the ... -
The biology, diversity and evolution of the broad host-range, promiscuous INCQ plasmids, with an emphasis on the INCQ2 sub-family
(Stellenbosch : Stellenbosch University, 2014-12)ENGLISH ABSTRACT: Plasmids belonging to the IncQ family have an exceptionally broad host-range and are highly mobilizable in the presence of the self-transmissible IncP plasmids. All IncQ plasmids identified to date have ... -
Bioprospecting for novel biosurfactants and biosurfactant producing bacteria in wastewater
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Biosurfactants are surface active amphiphilic compounds, synthesised by numerous bacteria, fungi and yeast. They are known to exhibit broad spectrum antimicrobial activity and are currently applied as ... -
Carnitine in yeast and filamentous fungi
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: In the yeast Saccharomyces cerevtstee, two biochemical pathways ensure that activated cytoplasmic or peroxisomal acetyl-groups are made available for mitochondrial energy production when the cells ... -
Characterisation of L-malic acid metabolism in strains of Saccharomyces and the development of a commercial wine yeast strain with an efficient malo-ethanolic pathway
(Stellenbosch : Stellenbosch University, 2002-12)ENGLISH ABSTRACT: L-Malic and tartaric acid are the most prominent organic acids in wine and playa crucial role in winemaking processes and wine quality, including the organoleptic quality and the physical, biochemical ... -
Characterisation of the malate transporter and malic enzyme from Candida utilis
(Stellenbosch : University of Stellenbosch, 2011-10)ENGLISH ABSTRACT: Yeast species differ remarkably in their ability to degrade extracellular dicarboxylic acids and to utilise them as their only source of carbon. The fission yeast Schizosaccharomyces pombe effectively ... -
Characterisation, cloning and heterologous expression of the α-glucuronidase from Aureobasidium pullulans
(Stellenbosch : Stellenbosch University, 2004-03)ENGLISH ABSTRACT: Xylanolytic accessory enzymes produced by the endo-p-l,4-xylanase overproducing, colour-variant strain of the euascomycetous fungus Aureobasidium pullulans, NRRL Y-2311-1, were studied. a- Glucuronidase ... -
Characterization and evaluation of indigenous Saccharomyces cerevisiae strains isolated from South African vineyards
(Stellenbosch : Stellenbosch University, 1999-12)ENGLISH SUMMARY: The bioconversion of grape juice into wine by simply allowing the yeasts, associated with grape berries and winery equipment, to ferment the sugars to ethanol, carbon dioxide and other minor, but important ... -
Characterization of bacteriocin 423 produced by Lactobacillus pentosus
(Stellenbosch : Stellenbosch University, 2000-12)ENGLISH ABSTRACT: Worldwide, bacteriocins, particularly those produced by food-related lactic acid bacteria, are receiving attention due to the possible use of these peptides as natural preservatives in food, replacing ... -
Characterization of nisin F and its role in the control of respiratory tract and skin infections
(Stellenbosch : University of Stellenbosch, 2009-03)Multidrug resistant strains of Staphylococcus aureus is presenting an increasing threat, especially immune compromised individuals. Many of these strains have developed resistance to newly approved drugs such as ... -
Characterization of the adhesion genes of probiotic lactic acid bacteria
(Stellenbosch : Stellenbosch University, 2008-03)One of the key selection criteria for potential probiotics is the ability to adhere and colonise the host gastrointestinal tract (GIT). Probiotics compete for receptor sites at the host intestinal surface, preventing ... -
Community-level analysis of the microbiology in constructed wetlands treating distillery effluent
(Stellenbosch : University of Stellenbosch, 2006-04)ENGLISH ABSTRACT: Constructed wetlands have been widely used in the treatment of industrial and domestic wastewater to reduce biological and chemical oxygen demand (BOD and COD), to remove nitrate and enteric viruses as ... -
Comparative proteomic and genomic analysis of Flavobacterium johnsoniae-like biofilm, planktonic and agar surface-associated cells
(Stellenbosch : University of Stellenbosch, 2010-03)ENGLISH ABSTRACT: Pathogenic Flavobacterium spp. cause serious disease outbreaks in a variety of farmed fish, which lead to large economic losses in the aquaculture industry on an annual basis. The ability of Flavobacterium ... -
Development of an antimicrobial wound dressing by co-electrospinning bacteriocins of lactic acid bacteria into polymeric nanofibers
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: Skin is the largest organ in the human body and serves as a barrier that protects the underlying tissue of the host from infection. Injury, however, destroys this protective barrier and provides a perfect ... -
Development of improved α-amylases
(Stellenbosch : University of Stellenbosch, 2005-03)The technological advancement of modern human civilisation has, until recently, depended on extensive exploitation of fossil fuels, such as oil, coal and gas, as sources of energy. Over the last few decades, greater ... -
The effects of native Saccharomyces cerevisiae snare gene over expression on Heterologous cellulase secretion
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: The budding yeast Saccharomyces cerevisiae has been successfully utilized in several industrial sectors and has over the last decade emerged as a promising host for the production of valuable heterologous ... -
Engineering a fungal β-fructofuranosidase
(Stellenbosch : Stellenbosch University, 2015-04)ENGLISH ABSTRACT: β-fructofuranosidases are hydrolytic enzymes that act on sucrose to yield the products glucose and fructose. Under high substrate conditions these enzymes display fructosyltransferase activity which results ... -
Engineering of Pichia stipitis for enhanced xylan utilization
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: Plant biomass, the most abundant renewable resource in nature, consists of matrices of mainly lignin, cellulose, hemicellulose as well as inorganic components. Xylan, the major hemicellulose component ... -
Enhancing xylose utilisation during fermentation by engineering recombinant Saccharomyces cerevisiae strains
(Stellenbosch : Stellenbosch University, 2007-12)ENGLISH ABSTRACT: Xylose is the second most abundant sugar present in plant biomass. Plant biomass is the only potential renewable and sustainable source of energy available to mankind at present, especially in the ... -
Evaluation of Aspergillus as a host for the production of viral proteins using hepatitis B as a model
(Stellenbosch : Stellenbosch University, 2002-12)ENGLISH ABSTRACT: Since the advent of recombinant DNA technology in the 1970s, various microbial hosts have been employed for the efficient high-level heterologous production of a variety of proteins, ranging from enzymes ...
