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Physiological induction of the acrosome reaction in human sperm: Validation of a microassay using minimal volumes of solubilized, homologous zona pellucida

Franken D.R.; Bastiaan H.S.; Oehninger S.C. (2000)

Purpose: To develop a method that could accommodate microvolumes of solubilized human zona pellucida (ZP) and sperm for assessing the induction of the acrosome reaction. Methods: A microassay using 1 μl of 2.5, 1.25, 0.6, ...