Now showing items 21-30 of 485
Use of failed-fertilized oocytes for diagnostic zona binding purposes after sperm binding improvement with a modified medium
Purpose: Because the availability of prophase oocytes for zona binding testing is limited, we compared sperm binding to the zona of failed- fertilized intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) ...
Defining bioassay conditions to evaluate sperm/zona interaction: Inhibition of zona binding mediated by solubilized human zona pellucida
Objective: Our goal was to study the influence of solubilized human zonae pellucidae on zona binding potential and acrosome reaction. Materials and Methods: Zona pellucida (ZP) solutions were prepared by dissolving zona ...
Physiological induction of the acrosome reaction in human sperm: Validation of a microassay using minimal volumes of solubilized, homologous zona pellucida
Purpose: To develop a method that could accommodate microvolumes of solubilized human zona pellucida (ZP) and sperm for assessing the induction of the acrosome reaction. Methods: A microassay using 1 μl of 2.5, 1.25, 0.6, ...
Seasonal changes in human sperm chromatin condensation
Purpose: The aim of this study was to investigate possible seasonal changes in human sperm parameters, especially chromatin condensation. Method: In a first run, 3155 patients attending the andrological outpatient clinic ...
An intracytoplasmic sperm injection pregnancy with a globozoospermic male
[No abstract available]
Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures a prospective, controlled, randomized study of four hundred and fifty patients
Background: The treatment of open fractures of the tibial shaft is often complicated by delayed union and non-union. The objective of this study was to evaluate the safety and efficacy of the use of recombinant human bone ...
Pyridoxal-5-phosphate plasma concentrations in children receiving tuberculosis chemotherapy including isoniazid
Aim: Little is known about pyridoxine nutriture of children treated with isoniazid (INH) regimens. This study documents plasma pyridoxal 5′-phosphate (PLP) concentrations in children, HIV-infected and HIV-uninfected, ...
Traumatic dislocation of the atlanto-occipital joint
We present four patients who had sustained a traumatic dislocation of the atlanto-occipital joint. The diagnosis was initially missed in two patients. One patient, who was neurologically intact, was treated non-operatively. ...
Childhood drug-resistant tuberculosis in the Western Cape Province of South Africa
Aim: Continued surveillance of drug resistance amongst children presenting with culture-confirmed tuberculosis to a tertiary care hospital and to community clinics. Methods: Drug susceptibility testing for isoniazid and ...
Capillary refilling time in newborns - Optimal pressing time, sites of testing and normal values
Aspects of capillary refilling time (CRT) in newborns, such as pressing time, sites of testing, normal values and difference between observers were assessed. CRT was measured in 280 term newborns, divided into 7 groups of ...