ITEM VIEW

A rapid high-performance liquid chromatography (HPLC) method for the extraction and quantification of folates in dairy products and cultures of Propionibacterium freudenreichii

dc.contributor.authorvan Wyk J.
dc.contributor.authorBritz T.J.
dc.date.accessioned2012-04-12T08:18:57Z
dc.date.available2012-04-12T08:18:57Z
dc.date.issued2012
dc.identifier.citationAfrican Journal of Biotechnology
dc.identifier.citation11
dc.identifier.citation8
dc.identifier.citation2087
dc.identifier.citation2098
dc.identifier.issn16845315
dc.identifier.other10.5897/AJB11.2491
dc.identifier.urihttp://hdl.handle.net/10019.1/20515
dc.description.abstractNutritional folate deficiencies in Southern African communities necessitated mandatory fortification. Current microbiological assays (MA) used to measure food folates, essential for quality control and regulatory purposes, are time-consuming. This study describes an alternative extraction and detection method for folates in dairy products and Propionibacterium freudenreichii cultures. Folates were extracted by heating with a phosphate buffer (pH 6.0). Polyglutamates were deconjugated with chicken pancreas and hog kidney deconjugases. Samples were purified using strong anion exchange solid phase extraction. Reversed-phase high-performance liquid chromatography (HPLC) using an acetonitrile-phosphate buffer (pH 2.2) gradient effectively separated four vitamers. Fluorescence (tetrahydrofolate (THF), 5-CH 3-THF and 5-CHO-THF), and UV detection (folic acid) were used, calibration curves were linear (R 2>0.0997), and detection and quantification limits were 0.0006 to 0.015 and 0.002 to 0.05 μg/ml, respectively. Accuracy was 80 to 108% and intra- and inter-day precision [%relative standard deviation (%RSD)] were lower than 4%. The method, validated against the standard MA, is a selective, sensitive, reliable and rapid alternative. © 2012 Academic Journals.
dc.subjectFolate
dc.subjectHigh-performance liquid chromatography (HPLC)
dc.subjectMicrobiological folate assay
dc.subjectPropionibacterium freudenreichii
dc.titleA rapid high-performance liquid chromatography (HPLC) method for the extraction and quantification of folates in dairy products and cultures of Propionibacterium freudenreichii
dc.typeArticle


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

ITEM VIEW