Determination of plasma amino acids by gas chromatography
Date
1986
Authors
Labadarios D.
Shephard G.S.
Botha E.
Journal Title
Journal ISSN
Volume Title
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Abstract
A complete methodology, incorporating a novel clean-up technique, for quantitative determination of amino acids in plasma by gas chromatography is described. Glucose, a component causing major analytical interference, is removed by an enzymic reaction included in the pre-chromatographic clean-up. The procedure for derivatisation of amino acid standards is shown to be reproducible down to a level of 2.5 μg for each amino acid, relative standard deviations for all amino acids except arginine and histidine being 3% or lower. For the entire procedure applied to plasma, relative standard deviations for most amino acids below 5% with recoveries ranging from 90 to 120%. Normal values, obtained using eighteen plasma samples, are in reasonable agreement with published data. Plasma amino acid values were determined simultaneously by gas chromatographic and ion-exchange chromatographic techniques. Statistical evaluation shows there to be no significant difference between corresponding values for eleven amino acids. Values for tyrosine, histidine and particularly phenylalanine show significant differences (p < 0.001).
Description
Keywords
amino acid, blood and hemopoietic system, gas chromatography, human, human cell, methodology, plasma, priority journal, Adenosine Triphosphate, Amino Acids, Chromatography, Gas, Chromatography, Ion Exchange, Hexokinase, Human, Indicators and Reagents
Citation
Journal of Chromatography - Biomedical Applications
383
2
383
2