In vitro effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential and motility of human spermatozoa
Date
2008
Authors
Falzone N.
Huyser C.
Fourie F.
Toivo T.
Leszczynski D.
Franken D.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Ejaculated, density purified, human spermatozoa were exposed to pulsed 900 MHz GSM mobile phone radiation at two specific absorption rate levels (SAR 2.0 and 5.7 W/kg) and compared with controls over time. Change in sperm mitochondrial membrane potential was analysed using flow cytometry. Sperm motility was determined by computer assisted sperm analysis (CASA). There was no effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential. This was also the case for all kinematic parameters assessed at a SAR of 2.0 W/kg. However, over time, the two kinematic parameters straight line velocity (VSL) and beat-cross frequency (BCF) were significantly impaired (P < 0.05) after the exposure at SAR 5.7 W/kg and no exposure by time interaction was present. This result should not be ascribed to thermal effects, due to the cooling methods employed in the RF chamber and temperature control within the incubator. © 2007 Wiley-Liss, Inc.
Description
Keywords
article, computer analysis, flow cytometry, human, human cell, kinematics, male, mitochondrial membrane potential, mobile phone, normal human, priority journal, radiation absorption, radiation exposure, radiation response, semen analysis, spermatozoon motility, thermoregulation, Cells, Cultured, Cellular Phone, Dose-Response Relationship, Radiation, Humans, Membrane Potential, Mitochondrial, Microwaves, Radiation Dosage, Sperm Motility
Citation
Bioelectromagnetics
29
4
29
4