Correlation between p53 gene mutation, p53 protein labeling and PCNA expression in oral squamous cell carcinomas
Date
1998
Authors
Van Heerden W.F.P.
Van Rensburg E.J.
Hemmer J.
Raubenheimer E.J.
Engelbrecht S.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: The prevalence of oral squamous cell carcinoma (OSCC) among the Black community in South Africa is unacceptably high. The association between p53 protein, and PCNA overexpression and the presence of p53 gene mutations was evaluated. Materials and Methods: One hundred and ten formalin-fixed, paraffin-embedded blocks of OSCC were selected for immunohistochemical studies for p53 protein and PCNA expression using the DO-7 and PC10 monoclonal antibodies, respectively. DNA was extracted from fifty-five blocks and exons 5 to 9 of the p53 gene were amplified with nested primers, thereafter sequencing was performed to confirm the presence of mutations detected by single stranded conformational polymorphism. Results: Fifty-six cases (51%) showed p53 expression, while fourteen mutations (25%) were detected. A significant difference was found between the PCNA index in p53 positive and p53 negative tumors while the mean PCNA index for the tumors with p53 mutations was not significantly different from the tumors without mutations. Conclusions: No association between p53 protein overexpression and p53 gene mutations could be demonstrated.
Description
Keywords
cycline, monoclonal antibody, protein p53, adult, aged, article, cancer genetics, dna determination, exon, female, frameshift mutation, gene amplification, gene mutation, gene overexpression, genetic association, human, human tissue, immunohistochemistry, major clinical study, male, mouth carcinoma, negro, priority journal, single strand conformation polymorphism, south africa, squamous cell carcinoma, Adult, African Continental Ancestry Group, Aged, Carcinoma, Squamous Cell, Cell Nucleus, Female, Genes, p53, Humans, Immunohistochemistry, Male, Middle Aged, Mouth Neoplasms, Point Mutation, Proliferating Cell Nuclear Antigen, Sequence Deletion, Tumor Suppressor Protein p53
Citation
Anticancer Research
18
1 A
18
1 A