Defective sperm decondensation: A cause for fertilization failure
Date
2002
Authors
Esterhuizen A.D.
Franken D.R.
Becker P.J.
Lourens J.G.H.
Muller I.I.
Van Rooyen L.H.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The study aimed to evaluate the role of chromatin packaging (CMA3 staining), sperm morphology during sperm-zona binding, sperm decondensation and the presence of polar bodies in oocytes that failed in vitro fertilization (IVF). The percentage CMA3 staining categorized the data into three groups, <44%, n=10; ≥44-59%, n=10; and ≥60%, n=29. Morphology groups were ≤4% (n=11); >4-14% (n=19); and >14% (n=19). One hundred and seventy-two oocytes that failed IVF were evaluated for sperm-zona binding, ooplasma penetration and sperm decondensation. Odds ratio analyses indicated that being in the ≥60% CMA3 staining group resulted in a 15.6 fold increase in the risk of decondensation failure, relative to CMA3 staining of <44%. For morphology, there was a 2.17 fold decrease in the risk of fertilization failure in the morphology group with >4-14% normal cells, while it increased 2.45 fold for the morphology group with ≤4% normal cells. Using CMA3 fluorescence to discriminate, 51% of the oocytes in the group with elevated CMA3 fluorescence had no sperm in the ooplasma compared to 32% and 16% penetration failure in the CMA3 staining groups ≥44-59% and <44%, respectively. Sperm chromatin packaging quality and sperm morphology assessments are useful clinical indicators of human fertilization failure. Immunofluorescence techniques could be used to provide a clear diagnosis of failed fertilization.
Description
Keywords
adult, article, binding affinity, cell structure, chromatin, controlled study, female, fertilization in vitro, human, human cell, immunofluorescence, major clinical study, male, oocyte, ovulation induction, spermatozoon, spermatozoon abnormality, spermatozoon penetration, staining, zona pellucida, Chromatin, Female, Fertilization in Vitro, Fluorescent Antibody Technique, Humans, Infertility, Male, Male, Microscopy, Fluorescence, Odds Ratio, Sperm Injections, Intracytoplasmic, Spermatozoa, Staining and Labeling, Treatment Failure
Citation
Andrologia
34
1
34
1